|本期目录/Table of Contents|

[1]黄家宝,刘翼翔,谢荔朋.利用物化-酶联工艺技术制备低分子质量的岩藻聚糖[J].集美大学学报(自然科学版),2026,31(5):540-551.
　HUANG Jiabao,LIU Yixiang,XIE Lipeng.Preparation of Low Molecular Weight Fucoidans by Physicochemical Enzyme Combined Technology[J].Journal of Jimei University,2026,31(5):540-551.
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《集美大学学报（自然科学版）》[ISSN:1007-7405/CN:35-1186/N]

卷:
第31卷
期数:
2026年第5期
页码:
540-551
栏目:
水产养殖与食品工程
出版日期:
2026-09-28

文章信息/Info

Title:
Preparation of Low Molecular Weight Fucoidans by Physicochemical Enzyme Combined Technology
作者:
黄家宝1; 刘翼翔1; 谢荔朋2
（1.集美大学海洋食品与生物工程学院，福建 厦门 361021；2.福建九为生物技术有限公司，福建 漳州 363900）
Author(s):
HUANG Jiabao1; LIU Yixiang1; XIE Lipeng2
(1.College of Ocean Food and Biological Engineering,Jimei University,Xiamen 361021,China;2.Fujian Jiuwei Biotechnology Co.,Ltd.,Zhangzhou 363900,China)
关键词:
九孔鲍; 岩藻聚糖裂解酶; 岩藻聚糖; 超声辅助; 高温高压
Keywords:
Haliotis diversicolor supertexta; fucoidan lytic enzyme; fucoidan; ultrasoundassisted; hightemperature/high-pressure
分类号:
-
DOI:
-
文献标志码:
A
摘要:
为构建兼具高水解效率与硫酸基团高保留度的岩藻聚糖降解工艺，本研究采用九孔鲍鱼（Haliotis diversicolor supertexta）肝胰腺制备岩藻聚糖裂解酶，通过高温高压等预处理技术，促进裂解酶与底物糖苷键的相互作用，提高低分子质量岩藻聚糖的产率并实现活性硫酸基团的有效保留。结果表明：无预处理下采用裂解酶水解2 h，岩藻聚糖溶液的相对黏度仅降低(15.13±2.37)%；超声（40 kHz）辅助HCl/H2O2预处理技术能使多糖溶液的相对黏度降低(20.6±1.37)%，而硫酸基团的保留度仅为(73.18±2.44)%；采用110 ℃、0.08 MPa的高温高压预处理5 min，岩藻聚糖水溶液的相对黏度下降(36.04±0.17)%，硫酸基团保留度为91.5%。岩藻聚糖经高温高压预处理后，采用裂解酶酶解90 min，其相对黏度降低了(79.37±1.64)%，硫酸基团保留度仍维持在91%以上。综上，高温高压酶联工艺可实现硫酸多糖的高效水解，并有效保留其硫酸基团，具有良好的产业化应用潜力。
Abstract:
To construct a fucoidan depolymerization process with both high hydrolysis efficiency and high sulfate group retention,the fucoidan lytic enzyme prepared from the hepatopancreas by-product of abalone (Haliotis diversicolor supertexta) was used as a tool enzyme,and high-temperature/high-pressure pretreatment techniques were combined to promote the interaction of the lytic enzyme with the glycosidic bond of the substrate,to achieve the effective retention of the activated sulfate group and substantially increase the yield of low molecular fucoidan.The results showed that the relative viscosity of the fucoidan solution reduced by only (15.13±2.37)% after enzymatic hydrolysis for 2 h without pretreatment;Ultrasonic (40 kHz)assisted HCl/H2O2 pretreatment effectively decreased the viscosity (20.6±1.37)%,whereas the retention rate of sulfate group was only (73.18±2.44)%;Hydrothermal pretreatment was performed at 110 ℃ and 008 MPa for 5 min,the viscosity of the aqueous fucoidan solution decreased by (3604±017)%,with a sulfategroup retention rate of 915%.After fucoidan was subjected to hydrothermal pretreatment,it was hydrolyzed with lyase for 90 min,its relative viscosity decreased by (79.37±1.64)%,and the retention rate of sulfate groups remained above 91%.Therefore,the hightemperature/highpressureenzymatic process technology can realize efficient hydrolysis of active sulfated polysaccharides,while effectively preserving sulfate groups,exhibiting great potential for industrial application.

参考文献/References:

相似文献/References:

[1]赵春暖,严正凛.皱纹盘鲍与九孔鲍杂交的研究[J].集美大学学报(自然科学版),2014,19(5):339.
　ZHAO Chun-nuan,YAN Zheng-lin.Hybridization Between Haliotis discus hannai and Haliotis diversicolor aquatilis[J].Journal of Jimei University,2014,19(5):339.

备注/Memo

备注/Memo:
更新日期/Last Update: 2026-11-01