高亲水性海参肽纯化工艺优化及其保湿能力-《集美大学学报（自然科学版）》

文章信息/Info

Title:: Research on the Purification Process Optimization of Highly Hydrophilic Sea Cucumber Peptides and Their Moisturizing Ability

作者:: 郝良兴1; 陈晖2; 3; 陈思谨2; 3; 谢婷玉2; 阎光宇3; 洪专2; 3; 张玉苍1; （1.集美大学海洋食品与生物工程学院，福建厦门 361021；2.自然资源部第三海洋研究所海洋生物资源开发利用工程技术创新中心，福建厦门 361005；3.厦门海洋职业技术学院海洋生物学院，福建厦门 361102）

Author(s):: HAO Liangxing1; CHEN Hui2; 3; CHEN Sijin2; 3; XIE Tingyu2; YAN Guangyu3; HONG Zhuan2; 3; ZHANG Yucang1; （1.College of Ocean Food and Biological Engineering,Jimei University,Xiamen 361021,China；2.Technology Innovation Center for Exploitation of Marine Biological Resources,Third Institute of Oceanography,Ministry of Natural Resources,Xiamen 361005,China;3.School of Marine Biology,Xiamen Ocean Vocational College,Xiamen 361102,China）

Keywords:: sea cucumber; sea cucumber peptide; ammonium sulfate precipitation; hydrophilicity; moisturizing ability

摘要:: 以北部湾低值黄玉参为原料，利用酶解法制备海参肽，通过硫酸铵沉淀法纯化高亲水性海参肽。以保湿性为指标，确定酶解条件为菠萝蛋白酶50 ℃酶解1 h；以表面疏水性为指标，经过单因素实验与响应面实验优化，确定硫酸铵沉淀条件，即样品质量分数为20.6%、硫酸铵终质量分数为42%、反应温度为35.5 ℃，高亲水性海参肽得率为5.3%。保湿性实验结果表明，纯化所得海参肽在温度为25 ℃、湿度为43%条件下放置12 h，其保湿率为89.9%，显著高于纯化前样品（85%）、2%甘油（82.3%）和5%甘油（84.1%）（P＜0.05）

Abstract:: Using low-value yellow sea cucumber from Beibu Gulf as raw material,sea cucumber peptides were prepared by enzymatic hydrolysis,and high hydrophilicity sea cucumber peptides were purified by ammonium sulfate precipitation.Taking moisturizing ability as the evaluation index,the optimal enzymatic hydrolysis parameters were determined as follows:hydrolysis with bromelain at 50 ℃ for 1 h.With surface hydrophobicity as the indicator,single-factor experiments combined with response surface methodology were adopted to optimize the ammonium sulfate precipitation conditions.The optimized conditions were a sample mass fraction of 206%,a final ammonium sulfate mass fraction of 42%,and a reaction temperature of 35.5 ℃,yielding high hydrophilicity sea cucumber peptide at a rate of 5.3%.Moisture retention test demonstrated that after being stored for 12 h at 25 ℃ and 43% relative humidity,the purified sea cucumber peptides achieved a moisture retention rate of 89.9%.This value was significantly higher than the unpurified sample(85%),2% glycerol solution(82.3%)and 5% glycerol solution(84.1%) (P<0.05).