膨腹海马生长性状全基因组关联分析及候选基因的挖掘-《集美大学学报（自然科学版）》

文章信息/Info

Title:: Gene-Wide Association Study to Identify Candidate Genes for Growth Traits in Hippocampus abdominalis

作者:: 郑磊1; 2; 3; 梁建韬4; 左小玲4; 韦丽云4; 闫旭4; 卢柯宇4; 张敏琳4; 梁凯珊4; 涂传灯1; 2; 3; 何丽斌5; 郑乐云1; 2; 3; 王庆4; 1.厦门海洋职业技术学院海洋生物学院，福建厦门361102；2.海洋资源保护与生态治理福建省高等学校应用技术工程中心，福建厦门361102；3.厦门市智慧渔业重点实验室，福建厦门361102；4.华南农业大学海洋学院，广东广州 510642；5.福建省水产研究所，福建厦门 361012

Author(s):: ZHENG Lei1; 2; 3; LIANG Jiantao4; ZUO Xiaoling4; WEI Liyun4; YAN Xu4; LU Keyu4; ZHANG Minlin4; LIANG Kaishan4; TU Chuandeng1; 2; 3; HE Libin5; ZHENG Leyun1; 2; 3; WANG Qing4; 1.School of Marine Biology,Xiamen Ocean Vocational College,Xiamen 361102,China;2.Applied Technology Engineering Center of Fujian Provincial Higher Education for Marine Resource Protection and Ecological Governance,Xiamen 361102,China;3.Xiamen Key Laboratory of Intelligent Fishery,Xiamen 361102,China;4.College of Marine Sciences,South China Agricultural University,Guangzhou 510642,China;5.Fisheries Research Institute of Fujian,Xiamen 361012,China

Keywords:: Hippocampus abdominalis; growth trait; genome-wide association study; single nucleotide polymorphism; candidate genes

摘要:: 传统的膨腹海马（Hippocampus abdominalis）生长性状的选育方法研究进展较慢，育种周期长，结合分子育种技术可加快育种进程。但是目前关于膨腹海马生长性状相关的分子标记和基因还不清楚。采集相同条件下极端快速生长（79尾）和极端缓慢生长（75尾）的膨腹海马，通过基因组重测序检测到4 172 760个单核苷酸多态性（single nucleotide polymorphism，SNP）位点，并通过全基因组关联分析（genome-wide association study，GWAS）鉴定出27个与生长性状关联的SNP位点，它们主要分布于6号染色体上，显著关联SNP对性状的解释贡献率合计达49.53%。此外，通过挖掘SNP位点上下游50 kb区域，找到42个潜在影响生长的候选基因，基因本体（gene ontology，GO）、京都基因与基因组百科全书（Kyoto encyclopedia of genes and genomes，KEGG）富集分析显示，这些基因主要富集在碳水化合物消化吸收、矿物质吸收和细胞连接等相关通路。进一步分析基因在快速生长个体和缓慢生长个体中的差异表达情况，发现15个基因可能与膨腹海马生长性状相关。

Abstract:: The traditional breeding methods for growth traits in Hippocampus abdominalis exhibit slow progress and a prolonged breeding cycle.The integration of molecular breeding techniques can expedite the breeding process.However,the molecular markers and genes associated with growth traits in Hippocampus abdominalis remain unclear.Here,big-belly seahorse individuals exhibiting extremely fast-growing (79 individuals) and extremely slow-growing (75 individuals) were collected under identical rearing conditions.A total of 4 172 760single nucleotide polymorphism（SNP）sites were identified by genome resequencing.Using genome-wide association study (GWAS),27 growth-related SNPs were detected，and predominantly located on chromosome 6These significantly associated SNPs collectively explained 49.53% of the total phenotypic variation.Furthermore,42 candidate genes potentially influencing growth were identified within 50 kb upstream and downstream regions surrounding SNP loci.Gene ontology（GO）and Kyoto encyclopedia of genes and genomes（KEGG） enrichment analysis revealed that these genes were primarily enriched in pathways related to carbohydrate digestion and absorption,mineral absorption,and cell junctions.Further investigation into the differential expression of genes between fastgrowing and slow-growing individuals indicated that 15 genes might be associated with growth traits in Hippocampus abdominalis.