藻油中DHA-EE的HPLC快速定量方法的建立-《集美大学学报（自然科学版）》

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作者:: 谢枫才1; 2; 张宾1; 孙继鹏2; (1.浙江海洋大学食品与医药学院,浙江舟山 316022;2.国家海洋局第三海洋研究所,国家海洋局海洋生物资源综合利用工程技术研究中心,福建厦门 361005)

Author(s):: XIE Fengcai1; 2; ZHANG Bin1; SUN Jipeng2; (1.College of Food Science and Pharmacy,Zhejiang Ocean University,Zhoushan 316022,China;2.Third Institute of Oceanography,State Oceanic Administration,Engineering Research Center of Marine Biological Resource Comprehensive Utilization，Xiamen 361005,China)

摘要:: 建立高效液相色谱法（high performance liquid chromatography，HPLC）快速测定藻油中二十二碳六烯酸乙酯（docosahexaenoic acid ethyl ester,DHA-EE）含量的方法。将藻油经乙酯化处理后，采用优化后DHA-EE分析参数：色谱柱ZORBAX SB-C8（4.6 mm×250 mm），流动相V(甲醇)∶V(水)=90∶10，柱温为30 ℃，流速为1.0 mL/min，紫外检测波长为204 nm，进样量为10 μL。测定结果显示，DHA-EE在0.008 1~1.92 μg 范围内呈良好线性关系，线性回归方程为：y=39.91 x+765.11（R2=0.999 2），平均回收率为102.42%（n=5，RSD=2.25%），精密度（n=10，RSD=0.32%）较好，DHA-EE最低定量限达8.05 ng。表明，该方法可用于藻油及其制品中DHA-EE的检测分析。

Abstract:: High performance liquid chromatography（HPLC）method was developed to quantify DHA-EE in marine microalgae oil quickly.The optimized quality parameters for DHA-EE analysis were as follows:chromatographic column,ZORBAX SB-C8（4.6 mm×250 mm）;mobile phase,methanol versus water ( 90∶10);the column temperature 30 ℃;the flow velocity 1.0 mL/min;UV wavelength 204 nm;the injection volume 10 μL.It presented a good linear relationship within range of 0.008 1-1.92 μg for DHA-EE,and the linear regression equation was y=39.91 x+765.11(R2=0.999 2). The average recovery rate was 102.42% (n=5,RSD=2.25%) with good precision(n=10,RSD=0.32%),and the minimum quantify limit reached 8.05 ng.This optimized HPLC method could be used in the determination and analysis of algal oil and DHA-EE