采用HS-SPME-GC-MS分析蜂蜜中的5-羟甲基糠醛衍生物-《集美大学学报（自然科学版）》

文章信息/Info

Title:: Analysis of Hydroxymethyl Furfural Derivatives in Honeys by HS-SPME-GC-MS

作者:: 陈路芳1; 2; 李丹丹1; 倪辉1; 2; 杨远帆1; 2; （1.集美大学海洋食品与生物工程学院，福建厦门 361021；2.厦门市食品生物工程技术研究中心，福建厦门 361021）

Author(s):: CHEN Lufang1; 2; LI Dandan1; NI Hui1; 2; YANG Yuanfan1; 2; (1.College of Ocean Food and Biological Engineering,Jimei University,Xiamen 361021,China;2.Research Center of Food Biotechnology of Xiamen City,Xiamen 361021,China）

Keywords:: 5-hydroxymethylfurfural derivatives; honey; head space solid phase micro-extraction; gas chromatography-mass spectrometry

摘要:: 以3种原蜜及其加热浓缩蜂蜜为实验材料，利用顶空固相微萃取联动气质色谱（head space solid phase micro-extraction gas chromatographymass spectrometry，HS-SPME-GC-MS）分析技术对比分析其中的5-羟甲基糠醛衍生物的含量。结果表明，利用HS-SPME-GC-MS方法从蜂蜜样品中鉴定出3种5-羟甲基糠醛衍生物；3种物质在质量浓度范围内均呈良好的线性关系，相关系数r2>0.990；检出限低于3.33×10-2 μg/L，定量限低于3.33 μg/L，加标回收率在80.76%～115.5%。研究发现，糠醛在3种加热浓缩蜂蜜中的含量显著高于对应的3种原蜜，而3-甲基-2-戊酮及2-乙基-1-己醇含量在3种加热浓缩蜂蜜与对应原蜜之间没有显著差异。说明，采用HS-SPME-GC-MS法测定蜂蜜中的糠醛含量可以区分原蜜及加热浓缩的蜂蜜。

Abstract:: In this study,three kinds of raw honey,as well as their heated and concentrated products were used as experimental materials to analyze the content of 5-hydroxymethylfurfural (5-HMF) derivatives by head space solid phase micro-extraction gas chromatographymass spectrometry(HSSPME-GC-MS).The results showed that three 5-HMF derivatives were identified.All three derivatives showed a good linear relationship in the range of mass concentration,and the correlation coefficient is r2>0.990.The detection limit was lower than 3.33×10-2 μg/L,the quantification limit was lower than 3.33 μg/L,and the recovery rate were in the range of 80.76%-115.5%.The furfural content in three heated and concentrated honey was significantly higher than that in raw honey,while the contents of 3-methyl-2-pentanone and 2-ethyl-1-hexanol showed no significant difference between the concentrated honey and raw honey.This study indicated that determination of furfural content in honey by the HS-SPME-GC-MS method is able to distinguish raw honey from heated concentrated honey.