|本期目录/Table of Contents|

[1]林文燕,翁朝红,杨江东,等.中国鲎同工酶组织器官特异性表达[J].集美大学学报(自然科学版),2010,15(5):333-337.
　LIN Wen-yan,WENG Zhao-hong,YANG Jiang-dong,et al.The Specific Expression of Isozymes in Different Tissues of Chinese Horseshoe Crab(Tachypleus tridentatus)[J].Journal of Jimei University,2010,15(5):333-337.
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《集美大学学报（自然科学版）》[ISSN:1007-7405/CN:35-1186/N]

卷:
第15卷
期数:
2010年第5期
页码:
333-337
栏目:
水产科学与生物工程
出版日期:
2010-09-25

文章信息/Info

Title:
The Specific Expression of Isozymes in Different Tissues of Chinese Horseshoe Crab(Tachypleus tridentatus)
作者:
林文燕1; 2; 翁朝红1; 2; 杨江东1; 2; 李伟文1; 2; 孙敏1; 2; 冯宇月1; 2
(1.集美大学水产学院,福建 厦门 361021;2.福建省高校水产科学技术与食品安全重点实验室,福建 厦门 361021)
Author(s):
LIN Wen-yan1; 2; WENG Zhao-hong1; 2; YANG Jiang-dong1; 2; LI Wei-wen1; 2;  SUN Min1; 2; FENG Yu-yue1; 2
(1.Fisheries College,Jimei University,Xiamen 361021,China;2.Key Laboratory of Science and Technology forAquaculture and Food Safety(Jimei University),Fujian Province,Xiamen 361021,China)
关键词:
中国鲎; 同工酶; 电泳; 组织特异性
Keywords:
Tachypleus tridentatus; isozyme; electrophoresis; tissue-specificity
分类号:
-
DOI:
-
文献标志码:
-
摘要:
］采用垂直聚丙烯酰胺凝胶电泳技术，分析了中国鲎6种组织器官（肝胰腺、心脏、肠、鳃、肌肉和黄色结缔组织）的乙醇脱氢酶（ADH）、山梨醇脱氢酶（SDH）、酯酶（EST）、过氧化氢酶（CAT）、天冬氨酸转化酶（AAT）、苹果酸脱氢酶（MDH）和苹果酸酶（ME）等7种同工酶的活性和分布，并对其酶谱的表型进行了分析.结果表明，中国鲎的同工酶系统存在不同程度的组织特异性.乙醇脱氢酶在鳃和黄色结缔组织中不表达；山梨醇脱氢酶在鳃中不表达；酯酶、过氧化氢酶和苹果酸脱氢酶在6种组织器官中均有表达，但表达活性存在差异；而天冬氨酸转化酶Aat-1位点在鳃中不表达
Abstract:
The activity，distribution and phenotype of seven isozymes such as alcohol dehydrogenase(ADH),sorbitol-dehydrogenase(SDH),esterase(EST),catalase(CAT),aspartate-aminotransferase(AAT),malate dehydrogenase(MDH) and malic enzyme(ME)in different tissues (hepatopancreas,heart,intestine,gill,muscle and yellow connective tissue) of Chinese horseshoe crab(Tachypleus tridentatus)were analyzed by using vertical polyacrylamide gel electrophoresis. The results showed that these isozymes presented tissues-specificity to some degree.ADH did not express in the gill and yellow connective tissue;SDH did not express in the gill;EST,CAT and MDH expressed in all tissues,but the level of activity in different tissues were variable;Aat-2 did not express in the gill

参考文献/References:

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更新日期/Last Update: 2014-06-28