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[1]陈俊,肖安风,倪辉,等.紫外分光光度法测定发酵液中麦考酚酸含量[J].集美大学学报(自然科学版),2011,16(4):258-262.
　CHEN Jun,XIAO An-feng,NI Hui,et al.Determination of Mycophenolic Acid in Fermentation Broth by UV Spectrophotometric Method[J].Journal of Jimei University,2011,16(4):258-262.
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《集美大学学报（自然科学版）》[ISSN:1007-7405/CN:35-1186/N]

卷:
第16卷
期数:
2011年第4期
页码:
258-262
栏目:
水产科学与生物工程
出版日期:
2011-07-25

文章信息/Info

Title:
Determination of Mycophenolic Acid in Fermentation Broth by UV Spectrophotometric Method
作者:
陈俊1; 2; 肖安风2; 3; 倪辉2; 3; 杨秋明2; 3; 蔡慧农2; 3
(1.华侨大学化工学院,福建 厦门361021;2.集美大学生物工程学院,福建 厦门 361021;3.厦门市食品生物工程技术研究中心,福建 厦门 361021)
Author(s):
CHEN Jun1; 2; XIAO An-feng2; 3; NI Hui2; 3; YANG Qiu-ming2; 3; CAI Hui-nong2; 3
(1.College of Chemical Engineering,Huaqiao University,Xiamen 361021,China;2.School of Biotechnology Engineering,Jimei University,Xiamen 361021,China;3.Food and Bio-engineering Technology Research Center of Xiamen,Xiamen 361021,China)
关键词:
短密青霉菌; 麦考酚酸; 紫外分光光度法; 发酵液
Keywords:
Penicillium brevicompactum; mycophenolic acid; UV spectrophotometeric method;  fermentation broth
分类号:
-
DOI:
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文献标志码:
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摘要:
分别采用乙醇、乙酸乙酯、甲醇3种有机溶剂提取发酵液中的麦考酚酸（MPA），分析紫外光谱吸收特征，并建立了测定MPA含量的紫外分光光度法.结果表明：MPA甲醇溶液质量浓度在0～50 mg/L范围内与Abs304呈良好的线性关系，平均回收率为1018 %，相对标准偏差为120 %.紫外分光光度法测定MPA含量简单、快速、重复性好，适用于发酵液中MPA含量的准确测定
Abstract:
A UV spectrophotometric method to determinine mycophenolic acid in zymotic fluid using ethanol,ethylacetate and methanol as extraction was established.The result showed:the linearity was in the range of 0～50 mg/L of MPA methanol solution,the average recovery was 101.8 %，and RSD was 1.20 %.The method was simple,rapid and reproducible,and it was suitable for the determination of mycophenolic acid in fermentation broth

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更新日期/Last Update: 2018-06-13