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[1]时超岚,曾润颖,曹敏杰,等.龙须菜寡糖分离纯化和抗食物过敏功效的研究[J].集美大学学报(自然科学版),2015,20(1):14-22.
　SHI Chao-lan,ZENG Run-ying,CAO Min-jie,et al.Research on the Purification and Anti-food Allergic Activity of Gracilaria lemaneiformis Oligosaccharide[J].Journal of Jimei University,2015,20(1):14-22.
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龙须菜寡糖分离纯化和抗食物过敏功效的研究(PDF)
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《集美大学学报（自然科学版）》[ISSN:1007-7405/CN:35-1186/N]

卷:
第20卷
期数:
2015年第1期
页码:
14-22
栏目:
水产、食品与生物工程
出版日期:
2015-01-25

文章信息/Info

Title:
Research on the Purification and Anti-food Allergic Activity of Gracilaria lemaneiformis Oligosaccharide
作者:
时超岚1; 2; 曾润颖3; 4; 曹敏杰1; 2; 刘庆梅1; 2; 张凌晶1; 2; 苏文金1; 2; 刘光明1; 2
1.集美大学食品与生物工程学院，福建 厦门 361021;2.福建省高校水产科学技术与食品安全重点实验室，福建 厦门 361021;3.国家海洋局第三海洋研究所，福建 厦门 361005;4.国家海洋局海洋生物遗传资源重点实验室，福建 厦门 361005
Author(s):
SHI Chao-lan1; 2; ZENG Run-ying3; 4; CAO Min-jie1; 2; LIU Qing-mei1; 2; ZHANG Ling-jing1; 2; SU Wen-jin1; 2; LIU Guang-ming1; 2
1.College of Food and Biological Engineering,Jimei University，Xiamen 361021,China;2.Key Laboratory of Science and Technology for Aquaculture and Food Safety,Fujian Province,Xiamen 361021,China;3.Third Institute of Oceanography，State Oceanic Administration，Xiamen 361005，China;4.Key Laboratory of Marine Biogenetic Resources，State Oceanic Administration,Xiamen 361005,China
关键词:
龙须菜; 寡糖; 纯化; 抗食物过敏; 原肌球蛋白; 小鼠模型; Th2免疫反应
Keywords:
Gracilaria lemaneiformis; oligosaccharide; purification; anti-food allergy; tropomyosin;  mouse model; Th2 immune response
分类号:
-
DOI:
-
文献标志码:
A
摘要:
为探究深海细菌Flammeovirga pacifica降解龙须菜所产生的寡糖抗食物过敏功效，以龙须菜为原料，通过发酵、超滤、Superdex Peptide HR 10/300柱层析等方法获得高纯度寡糖.离子色谱结果显示：分离纯化获得的寡糖主要为四糖和六糖，其质量分数分别为48.47%和25.69%.小鼠过敏模型实验结果表明，腹腔注射该寡糖（100 μg/只）能显著降低过敏小鼠血清特异性IgE和IgG1水平，提高特异性IgG2a水平.通过灌胃（10 mg/只）能显著降低小鼠粪便中组胺的水平，且能有效缓解空肠组织过敏病理现象.小鼠脾淋巴细胞实验结果显示，该寡糖能在转录和转译水平上显著降低Th2细胞因子（IL-4、IL-5、IL-13）含量，促进Th1细胞因子IFN-γ分泌.说明该寡糖能有效调节辅助性T细胞的免疫功能向Th1细胞转换，从而抑制Th2细胞主导的食物过敏反应.
Abstract:
In order to investigate the anti-food allergic effect of Gracilaria lemaneiformis oligosaccharides which were degraded by the deep sea bacteria Flammeovirga pacifica,the oligosaccharides were obtained from Gracilaria lemaneiformis through fermentation,ultrafiltration,and purified by Superdex peptide HR 10/300 chromatography column.The oligosaccharides obtained are mainly tetrasccharide and hexaose，their contents were 48.47% and 25.69% respectively as detected by ion chromatography.The results of the allergic mouse model indicated that intraperitoneal injection of Gracilaria lemaneiformis oligosaccharides (100 μg/mouse) significantly suppressed serum specific IgE,IgG1 level,and promoted specific IgG2a level in mice.Gavage of Gracilaria lemaneiformis oligosaccharides(10 mg/mouse) decreased fecal histamine release and repaired the pathology in the jejunum.The results of the mouse splenic lymphocytes experiment showed that Th2 cytokine (IL-4,IL-5 and IL-13) contents were reduced,while the Th1 cytokine (IFN-γ) content was up-regulated by Gracilaria lemaneiformis oligosaccharides in the transcription and translation levels.These results suggested that Gracilaria lemaneiformis oligosaccharides could cause the immune function of helper T cells to convert to the Th1,so as to inhibit the food allergies dominated by Th2 cells.

参考文献/References:

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相似文献/References:

[1]殷勤,肖安风,朱艳冰,等.酶解龙须菜粗多糖硫酸基工艺优化[J].集美大学学报(自然科学版),2015,20(3):173.
　YIN Qin,XIAO An??-feng,ZHU Yan-bing,et al.Optimization of Enzymatic Hydrolysis of Sulfate in Crude Polysaccharide from Gracilaria lemaneiformis by Recombinant Arylsulfatase[J].Journal of Jimei University,2015,20(1):173.
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备注/Memo

备注/Memo:
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更新日期/Last Update: 2015-03-05