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[1]王鹤,巫晶晶,张金丽,等.一株耐镍寡养单胞菌的分离及鉴定[J].集美大学学报(自然科学版),2015,20(3):186-192.
 WANG He,WU Jing-jing,ZHANG Jin-li,et al.Isolation and Characterization of a Nickel-resistant Stenotrophomonas Strain[J].Journal of Jimei University,2015,20(3):186-192.
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一株耐镍寡养单胞菌的分离及鉴定(PDF)
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《集美大学学报(自然科学版)》[ISSN:1007-7405/CN:35-1186/N]

卷:
第20卷
期数:
2015年第3期
页码:
186-192
栏目:
水产、食品与生物工程
出版日期:
2015-05-25

文章信息/Info

Title:
Isolation and Characterization of a Nickel-resistant Stenotrophomonas Strain
作者:
王鹤12巫晶晶12张金丽12王洁12贾成光12张亚平12
(1.集美大学食品与生物工程学院,福建 厦门 361021; 2.福建省食品微生物与酶工程重点实验室,福建 厦门 361021)
Author(s):
WANG He12WU Jing-jing12ZHANG Jin-li12WANG Jie12JIA Cheng-guang12ZHANG Ya-ping12
(1.College of Food and Biological Engineering,Jimei University,Xiamen 361021,China;2.Fujian Provincial Key Laboratory of Food Microbiology and Enzyme Engineering,Xiamen 361021,China)
关键词:
微生物筛选重金属耐受菌
Keywords:
microbialscreeningheavy metaltolerant bacteriaNi2+
分类号:
-
DOI:
-
文献标志码:
A
摘要:
从福建某五金厂综合污水中筛选到一株对Ni2+耐受性400 mg/L的菌株,对其形态学、16S rDNA序列及生理生化特征进行分析,并利用较佳生长条件对所得菌株进行Ni2+吸附验证.结果表明:该菌为革兰氏阴性杆菌,在牛肉膏蛋白胨培养基上形成的菌落,表面较光滑,边缘整齐;经16S rDNA序列比对及生理生化特征的分析,将菌株 JMUZJ-1鉴定为寡养单胞菌(Stenotrophomonas sp.);菌株JMUZJ-1生长的较好条件是pH 7.0,温度30 ℃,NaCl质量分数0.5%,在该条件下,菌株对Ni2+表现出良好的吸附性能.
Abstract:
A nickel resistant strain was obtained from a hardware factory in Fujian province by selection of spontaneous mutants in wastewater containing Ni2+ .Morphological tests,molecular identifications of 16S rDNA and biochemical characterization to this strain have been performed.The efficiency of the strain in absorbing Ni2+ under optimal growing conditions has been analyzed.The results showed that the nickel?-resistant strain was Gram-negative.The strain produces yellow pigmented colonies on medium including beef extract and peptone,with smooth surface and neat edge.Sequence analysis of 16S rDNA and biochemical characteristics identified the strain JMUI-1 as Stenotrophomonas sp.The optimal growth condition for the nickel resistant strain is at pH 7.0,30 ℃,containing 0.5% NaCl solution,which allows JMUI-1 a good absorption performance of Ni2+.

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更新日期/Last Update: 2015-07-04