|本期目录/Table of Contents|

[1]徐超,鲍俊旺,蔡秋凤,等.鲤鱼肌肉Ⅴ型胶原蛋白的分离纯化及其抗体制备[J].集美大学学报(自然科学版),2015,20(6):401-406.
　XU Chao,BAO Jun-wang,CAI Qiu-feng,et al.Purification of Type V Collagen from Common Carp(Cyprinus carpio)Muscle and Preparation of Polyclonal Antibody[J].Journal of Jimei University,2015,20(6):401-406.
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鲤鱼肌肉Ⅴ型胶原蛋白的分离纯化及其抗体制备(PDF)
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《集美大学学报（自然科学版）》[ISSN:1007-7405/CN:35-1186/N]

卷:
第20卷
期数:
2015年第6期
页码:
401-406
栏目:
水产、食品与生物工程
出版日期:
2015-11-25

文章信息/Info

Title:
Purification of Type V Collagen from Common Carp(Cyprinus carpio)Muscle and Preparation of Polyclonal Antibody
作者:
徐超1; 鲍俊旺1; 蔡秋凤1; 2; 张凌晶1; 2; 刘光明1; 2; 曹敏杰1; 2
(1.集美大学食品与生物工程学院，福建 厦门 361021；2.福建省水产品深加工工程研究中心，福建 厦门 361021)
Author(s):
XU Chao1; BAO Jun-wang1; CAI Qiu-feng1; 2; ZHANG Ling-jing1; 2; LIU Guang-ming1; 2; CAO Min-jie1; 2
(1.College of Food and Biological Engineering,Jimei University,Xiamen 361021,China;2.Engineering Research Center for Aquatic Products Processing of Fujian Province,Xiamen 361021,China)
关键词:
V型胶原蛋白; 圆二色谱; 多克隆抗体; 免疫印迹法; 鲤鱼
Keywords:
type V collagen; circular dichroism; polyclonal antibody; Western blotting; Cyprinus carpio
分类号:
-
DOI:
-
文献标志码:
A
摘要:
通过酸溶、胃蛋白酶酶解和氯化钠盐析等方法，从鲤鱼肌肉中纯化得到了V型胶原蛋白.SDS-PAGE电泳图谱分析发现，其至少由2条α链（α1和α2）组成，其中α1分子质量约为135 ku，α2分子质量约为120 ku.通过圆二色光谱仪分析其二级结构，发现其具有胶原蛋白三股螺旋结构的典型特征，其热变性温度为30.5 ℃.将V型胶原蛋白作为抗原免疫新西兰大白兔，成功制备了多克隆抗体.采用免疫印迹法对该多克隆抗体的特异性进行分析，证明其仅与鱼类V型胶原蛋白产生反应，特异性良好.
Abstract:
The type V collagen from common carp muscle was extracted by pepsin digestion and salt precipitation.SDS-PAGE analysis revealed that the protein contains at least two different α chains(α1 andα2).The molecular mass of α1chain was about 135 ku,while that of α2chain was around 120 ku.The characteristic of type V collagen is typical with triple helical structure and its denaturation temperature is 30.5 ℃ as assessed by circular dichroism.Purified type V collagen was used as an antigen to immunize a New Zealand rabbit subcutaneously to prepare a specific polyclonal antibody.The specificity of the anti-collagen(V)polyclonal antibody was confirmed by Western blotting and it specifically reacted with type V collagen from fish.

参考文献/References:

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相似文献/References:

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　LI Yue,YAN Longjie,WENG Ling,et al.Effect of Metal Ions on the Activity of a Prolyl Endopeptidase from Haliotis discus hannai Using Circular Dichroism Spectrum[J].Journal of Jimei University,2019,24(6):167.

备注/Memo

备注/Memo:
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更新日期/Last Update: 2015-12-16