|本期目录/Table of Contents|

[1]朱珊珊,翁朝红,肖世俊,等.美洲鲎miRNA的生物信息学挖掘与分析[J].集美大学学报(自然科学版),2016,21(2):107-114.
　ZHU Shan-shan,WENG Zhao-hong,XIAO Shi-jun,et al.Bioinformatic Identification of Conserved miRNA in Limulus polyphemus[J].Journal of Jimei University,2016,21(2):107-114.
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美洲鲎miRNA的生物信息学挖掘与分析(PDF)
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《集美大学学报（自然科学版）》[ISSN:1007-7405/CN:35-1186/N]

卷:
第21卷
期数:
2016年第2期
页码:
107-114
栏目:
水产、食品与生物工程
出版日期:
2016-03-28

文章信息/Info

Title:
Bioinformatic Identification of Conserved miRNA in Limulus polyphemus
作者:
朱珊珊1; 2; 翁朝红1; 2; 肖世俊1; 2; 韩兆方1; 2; 谢仰杰1; 2
（1.集美大学水产学院，福建 厦门 361021；2.农业部东海海水健康养殖重点实验室，福建 厦门 361021）
Author(s):
ZHU Shan-shan1; 2; WENG Zhao-hong1; 2; XIAO Shi-jun1; 2; HAN Zhao-fang1; 2; XIE Yang-jie1; 2
(1.Fisheries College，Jimei University，Xiamen 361021，China;2.Key Laboratory of Healthy Mariculture for the East China Sea，Ministry of Agriculture，Xiamen 361021，China)
关键词:
miRNA; 美洲鲎; 生物信息学; 挖掘
Keywords:
miRNA; Limulus polyphemus; bioinformatics; mining
分类号:
-
DOI:
-
文献标志码:
A
摘要:
利用miRBase数据库中已有动物的小RNA（miRNA），通过生物信息学方法对美洲鲎(Limulus polyphemus)表达序列标签（Expressed Sequences Tag,EST）和cDNA进行序列比对，挖掘美洲鲎miRNA，得到了 72个miRNA前体(pre-miRNA)、49个可编码成熟miRNA，隶属于40个miRNA家族。miRNA家族归类结果表明，无论在低等脊椎动物还是高等动物中，miRNA家族的存在都是相当保守的。以miR-10基因前体为例，分析其序列与其他物种同源序列的差异，显示miRNA序列的高度保守性。miR-10前体序列分析系统进化发现，物种聚类与传统分类亲缘关系一致。
Abstract:
In this study，bioinformatics method were used to identify microRNAs of Limulus polyphemus.The ESTs and cDNA sequences of L.polyphemus were aligned to the known animal miRNAs from miRBase.72 miRNA precursors (pre-miRNAs) and 49 mature miRNAs belonging to 40 miRNA families were identified.The classified analysis of miRNA family showed that mature miRNAs and precursor sequences are highly conserved，not only existing in the low class of invertebrates but also in high vertebrates.Analysis of sequences homology with other species based on miR-10 precursors indicated that the sequences of miRNA were fairly conserved.The phylogenetic analysis of miR-10 precursor sequences revealed that clustering mode was consistent with the traditional classification of related species.

参考文献/References:

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相似文献/References:

[1]房路京,肖世俊,王志勇.大黄鱼转录组水平miRNA的生物信息学分析[J].集美大学学报(自然科学版),2017,22(3):8.
　FANG Lujing,XIAO Shijun,WANG Zhiyong.Transcriptome-wide Bioinformatics Analysis of miRNA in Large Yellow Croaker Larimichthys crocea[J].Journal of Jimei University,2017,22(2):8.
[2]翁朝红,谢仰杰,肖志群,等.中国鲎保守miRNA靶基因预测及功能分析[J].集美大学学报(自然科学版),2017,22(6):1.
　WENG Zhaohong,XIE Yangjie,XIAO Zhiqun,et al.Target Genes Prediction and Their Functional Analysis of Conserved miRNAs from Tachypleus tridentatus[J].Journal of Jimei University,2017,22(2):1.

备注/Memo

备注/Memo:
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更新日期/Last Update: 2016-05-05