|本期目录/Table of Contents|

[1]刘萌,刘光明,刘翼翔,等.生物酶法制备海带多酚的工艺研究[J].集美大学学报(自然科学版),2017,22(1):21-28.
　LIU Meng,LIU Guangming,LIU Yixiang,et al.Extracting Polyphenols from Laminaria japonica Based on Bio-enzymes Extraction Method[J].Journal of Jimei University,2017,22(1):21-28.
点击复制

生物酶法制备海带多酚的工艺研究(PDF)
分享到：

《集美大学学报（自然科学版）》[ISSN:1007-7405/CN:35-1186/N]

卷:
第22卷
期数:
2017年第1期
页码:
21-28
栏目:
水产、食品与生物工程
出版日期:
2017-01-28

文章信息/Info

Title:
Extracting Polyphenols from Laminaria japonica Based on Bio-enzymes Extraction Method
作者:
刘萌1; 刘光明1; 2; 3; 4; 刘翼翔1; 2; 3; 4; 陈庆绸1; 邱绪建1; 孙乐常1; 杨燊1; 郝更新1
（1.集美大学食品与生物工程学院，福建 厦门 361021；2.厦门市海洋功能食品重点实验室，福建 厦门 361021；3.福建省海洋功能食品工程技术中心，福建 厦门 361021；4.水产品深加工技术国家地方联合工程研究中心，福建 厦门 361021）
Author(s):
LIU Meng1; LIU Guangming1; 2; 3; 4; LIU Yixiang1; 2; 3; 4; CHEN Qingchou1;  QIU Xujian1; SUN Lechang1; YANG Shen1; HAO Gengxin1
(1.College of Food and Biological Engineering,Jimei University,Xiamen 361021,China; 2.Xiamen Key Laboratory of Marine Functional Food,Xiamen 361021,China; 3.Marine Functional Food Engineering Technology Center of Fujian Province,Xiamen 361021,China; 4.National Joint Engineering Research Center for Deep Processing of Aquatic Products,Xiamen 361021,China)
关键词:
海带; 多酚; 生物酶; 固相萃取
Keywords:
Laminaria japonica; polyphenols; bio-enzymes; solid phase extraction
分类号:
-
DOI:
-
文献标志码:
A
摘要:
以海带为原料，采用酶法破壁技术和固相萃取技术，研究纤维素酶、果胶酶及其组成的复合酶对海带多酚溶出效果的影响，并确定最佳工艺条件。结果表明，选用果胶酶进行酶解破壁，最佳工艺条件为：加酶量20 000 U·kg-1，酶解pH值5.0，酶解温度60 ℃，酶解时间90 min，海带多酚提取率达1.68 g/kg；经固相萃取纯化后，海带多酚的高效液相色谱（high performance liquid chromatography,HPLC）信号值提高了9.24倍，杂质峰明显减少，实现了海带多酚的高效纯化。
Abstract:
Bio-enzymes and solid phase extraction were used for the extraction of polyphenols from kelp.The extraction efficiency for polyphenol extracts from Laminaria japonicaby using cellulase,pectinase and their compounds were evaluated and then the optimal technological conditions were determined.The results showed that the extraction efficiency of polyphenols was 1.68 g/kg after pectinase enzyme hydrolyzing the cell walls of Laminaria japonica;and the optimum parameters of enzyme hydrolyzing were as follows:enzyme hydrolyzing for 90 min, 2000 U·kg-1,pH 5.0,60 ℃,the signal value of high performance liquid chromatography(HPLC) was improved to 9.24 times and the impurity peaks were significantly reduced after solid phase extraction being employed.

参考文献/References:

-

相似文献/References:

[1]吴兰兰,汤凤霞,何传波,等.响应面法优化龙眼核多酚提取工艺的研究[J].集美大学学报(自然科学版),2010,15(5):342.
　WU Lan-lan,TANG Feng-xia,HE Chuan-bo,et al.Study on Extraction of Polyphenols from Longan Seed byResponse Surface Analysis[J].Journal of Jimei University,2010,15(1):342.
[2]欧俊新,严正凛.南方鲍藻混养技术研究及其效益分析[J].集美大学学报(自然科学版),2011,16(3):172.
　OU Jun-xin,YAN Zheng-lin.The Technology and Efficiency of MacrophyteHaliotis Mixed Breeding Mode in South China[J].Journal of Jimei University,2011,16(1):172.
[3]王翔宇,于晓清,丁刚,等.低温弱光保存的海带（Saccharina japonica）雌配子体细胞结构变化[J].集美大学学报(自然科学版),2019,24(1):14.
　WANG Xiangyu,YU Xiaoqing,DING Gang,et al.Study on Changes in Cell Structure of Saccharina japonica Female Gametophyte Under Low Temperature and Weak Light[J].Journal of Jimei University,2019,24(1):14.

备注/Memo

备注/Memo:
-
更新日期/Last Update: 2017-03-09