|本期目录/Table of Contents|

[1]孙莎,韩兆方,李完波,等.黄姑鱼（Nibea albiflora）gsdf基因的克隆及表达分析[J].集美大学学报(自然科学版),2018,23(2):81-90.
　SUN Sha HAN ZhaofangLI WanboYE KunLIN AiqiangCUI XiaoyingWANG Zhiyong.Cloning and Expression Analysis of gsdf Gene in the Yellow Drum(Nibea albiflora)[J].Journal of Jimei University,2018,23(2):81-90.
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《集美大学学报（自然科学版）》[ISSN:1007-7405/CN:35-1186/N]

卷:
第23卷
期数:
2018年第2期
页码:
81-90
栏目:
水产、食品与生物工程
出版日期:
2018-03-28

文章信息/Info

Title:
Cloning and Expression Analysis of gsdf Gene in the Yellow Drum(Nibea albiflora)
作者:
孙莎1; 2; 韩兆方1; 2; 李完波1; 2; 叶坤1; 2; 林爱强1; 2; 崔晓莹1; 2; 王志勇1; 2
(1.集美大学水产学院,福建 厦门 361021;2.农业部东海海水健康养殖重点实验室,福建 厦门 361021)
Author(s):
SUN Sha 12HAN Zhaofang12LI Wanbo12YE Kun12LIN Aiqiang12CUI Xiaoying12WANG Zhiyong12
(1.Fisheries CollegeJimei UniversityXiamen 361021China;2.Key Laboratory of Healthy Mariculture for the East China Sea of Ministry of Agriculture,Xiamen 361021China)
关键词:
黄姑鱼;  gsdf基因; 组织表达; 时序表达
Keywords:
yellow drumNibea albiflora; gsdf (gonadal soma derived factor); tissue expression pattern; temporal expression
分类号:
-
DOI:
-
文献标志码:
-
摘要:
］为探索gsdf（gonadal soma derived factor）基因对黄姑鱼性别分化和性腺发育的作用，克隆了黄姑鱼gsdf基因ORF序列全长，并运用实时荧光定量PCR（qRT-PCR）技术检测了该基因在黄姑鱼不同组织和不同发育时期性腺中的表达情况。结果显示：黄姑鱼gsdf基因开放阅读框长648 bp，可编码215个氨基酸，含有TGF-β超家族的保守功能结构域；组织表达分析结果显示，黄姑鱼gsdf基因主要集中在精巢中表达；在23 ℃左右水温下，孵化后40 d的遗传雄性鱼苗性腺中开始检测到gsdf基因有明显表达（此时组织学上尚看不出精巢形态），49 d后表达量迅速升高，孵化120 d后（精巢分化完成）表达量达到高峰，随后表达量开始下降，直到黄姑鱼精巢成熟之后呈现稳定表达。上述结果提示，黄姑鱼gsdf基因与黄姑鱼性别决定和分化发育过程密切相关。
Abstract:
In order to study the function of gsdf (gonadal soma derived factor) in sex differentiation and gonad development in the yellow drum（Nibea albiflora）,the ORF (open reading frame) sequence of gsdf gene was obtained through molecular and bioinformatics technique.And the expression profile of gsdf in different tissues and different development stages of gonads was assayed by qRT-PCR (quantitative real time PCR).Results showed that the gsdf contained a 648 bp open reading frame encoding a protein of 215 amino acids with a conserved domain shared by the TGF-β superfamily.The qRT-PCR analysis revealed that gsdf mainly expressed in testis of the yellow drum.Hatching and rearing at 23 ℃，the gsdf expression in the genetic males was detected from 40 dph (day post hatch) while the testis was unidentifiable by histology.The expression of gsdf went up rapidly after 49 dph，and reached to the maximum peak on 120 dph when the testis was fully differentiated.Subsequently，the expression of gsdf showed down regulation and it tended to be stabilized after the testis was fully mature.These results indicated that gsdf was tightly related to the testis differentiation and development in N.albiflora

参考文献/References:

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更新日期/Last Update: 2018-05-24