市售三种食用贝类可培养细菌多样性研究-《集美大学学报（自然科学版）》

文章信息/Info

Title:: Cultivable Bacterial Diversity of Three Kinds of Retail Edible Shellfish

作者:: 张媛1; 2; 徐晓津1; 2; 黄力行1; 2; 赵玲敏1; 2; 鄢庆枇1; 2; 3; （1.集美大学水产学院，农业部东海海水健康养殖重点实验室，福建厦门 361021；2.福建省天马科技股份有限公司，福建省特种水产饲料重点实验室，福建福州 350308）

Author(s):: ZHANG Yuan1; XU Xiaojin1; HUANG Lixing1; ZHAO Lingmin1; YAN Qingpi1; 2; (1.Fisheries College,Jimei University,Xiamen 361021,China;2.Key Laboratory of Healthy Mariculture for the East China Sea(Ministry of Agriculture),Xiamen 361021,China;3.Fujian Tianma Technology Company Limited & Key Laboratory of Special Aquatic Feed for Fujian,Fuzhou 350308,China)

Keywords:: Ostrea plicatula; Sinonovacula constricta; Thais clayigera; bacterial diversity; pathogenicity

摘要:: 为了解厦门市售三种食用贝类中可培养细菌的多样性，用自制培养基培养牡蛎、缢蛏、疣荔枝螺样品中的细菌，并根据16S rDNA测序结果，对所分离的细菌进行鉴定并进行系统发育分析。研究结果显示：从牡蛎中分离得到的35株细菌分属14个种，从缢蛏中分离得到的32株细菌分属16个种，从疣荔枝螺中分离得到的31株细菌分属7个种。结果表明市售牡蛎、缢蛏、疣荔枝螺体内含有较为丰富的细菌种类，而且分离得到的细菌（如解鸟氨酸拉乌尔菌、溶藻弧菌、副溶血弧菌等）大多数具有致病性，对食用人群具有潜在威胁，不建议直接生食或食用加工不当的贝类产品。

Abstract:: In order to understand the diversity of culturable bacteria in three types of edible shellfish commercially available in Xiamen,the bacteria isolated from Ostrea plicatula,Sinonovacula constricta and Thais clavigera were cultured with self??????-made medium.The species of bacteria were identified and phylogenetic analysis was carried out according to the results of 16S rDNA sequencing.The results showed that the 35 strains isolated from Ostrea plicatula belong to 14 species,the 32 strains isolated from Sinonovacula constricta belong to 16 species,and the 31 strains isolated from Thais clayigera belong to 7 species.The results indicated that commercially available oyster,sinonovacula and rock whelk are rich in bacteria,and most of the isolated bacteria (such as Raoultella ornithinolytica,Vibrio alginolyticus and Vibrio parahaemolyticus,etc.) were pathogenic.So it is not recommended to eat improperly processed shellfish products or the raw shell fish.