|本期目录/Table of Contents|

[1]王有朝,曹敏杰,苏文金,等.R-藻蓝蛋白的分离纯化及抗食物过敏活性研究[J].集美大学学报(自然科学版),2013,18(4):253-260.
　WANG You-zhao,CAO Min-jie,SU Wen-jin,et al.Research on the Purification and Anti-food Allergic Activity of R-phycocyanin[J].Journal of Jimei University,2013,18(4):253-260.
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《集美大学学报（自然科学版）》[ISSN:1007-7405/CN:35-1186/N]

卷:
第18卷
期数:
2013年第4期
页码:
253-260
栏目:
水产科学与生物工程
出版日期:
2013-07-25

文章信息/Info

Title:
Research on the Purification and Anti-food Allergic Activity of R-phycocyanin
作者:
王有朝1; 2; 曹敏杰1; 2; 苏文金1; 2; 张凌晶1; 2; 毛海燕1; 2; 时超岚1; 2; 刘光明1; 2
(1.集美大学生物工程学院,福建 厦门 361021 ;2.福建省高校水产科学技术与食品安全重点实验室,福建 厦门 361021)
Author(s):
WANG You-zhao1; 2; CAO Min-jie1; 2; SU Wen-jin1; 2; ZHANG Ling-jing1; 2; MAO Hai-yan1; 2; SHI Chao-lan1; 2; LIU Guang-ming1; 2
(1.College of Biological EngineeringJimei University,Xiamen 361021,China 2.Key Laboratory of Science andTechnology for Aquaculture and Food Safety (Jimei University )Fujian ProvinceXiamen 361021China)
关键词:
坛紫菜; R-藻蓝蛋白; 抗过敏活性; 食物过敏; 原肌球蛋白
Keywords:
Porphyra haitanensis; R-phycocyanin; anti-allergy activity; food allergy; tropomyosin
分类号:
-
DOI:
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文献标志码:
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摘要:
     为探究R-藻蓝蛋白抗过敏功效，以坛紫菜为原料，通过25%~35%硫酸铵盐析和DEAE-Sepharose Fast Flow 、Sephacryl S-200 HR 两步柱层析获得了高纯度的目的蛋白（A620/A280>6.0）.SDS-PAGE显示,该蛋白质由分子质量为17.7 ku和20.5 ku的两个亚基组成，紫外可见分光光度法测得其在620 nm、555 nm有两个特征吸收峰，可确认其为R-藻蓝蛋白.热稳定性和pH值稳定性实验结果显示，R-藻蓝蛋白在低于35 ℃、pH=40~9.0条件下光谱性质相对稳定.动物实验结果表明，100 μg/mL高纯度R-藻蓝蛋白能够显著降低小鼠血清中IgE水平；细胞实验结果表明，40 μg/mL R- 藻蓝蛋白能够降低细胞因子IL-4、IL-13的蛋白质分泌量；基因表达量分析结果显示，R-藻蓝蛋白能够降低IL-4在淋巴细胞中的表达量.可见，坛紫菜R-藻蓝蛋白通过促使CD4+T cell向TH1型转化发挥抗食物过敏活性
Abstract:
In order to explore the anti-food allergic effect of R-phycocyanin，high purity（A620/A280>6.0) of interest protein from Porphyra haitanensis was acquired after 25 %~35 % ammonium sulfate fractionation ，DEAE-Sepharose Fast Flow and Sephacryl S -200 HR column chromatographies.SDS-PAGE showed that the protein consisted of two different subunits，with molecular weight of 17.7 ku and 20 .5 ku,respectively.Two characteristic absorption peaks at 620 nm and 555 nm as detected by ultraviolet spectrophotometer were used to identify R -phycocyanin.Thermal and pH stability test demonstrated that the spectroscopic properties of R -phycocyanin was stable under the conditions of< 35 ℃，pH=4.0~9.0.Animal experiment showed that R-phycocyanin（100 μg/mL) could reduce the IgE level in mouse serum significantly.In vitro cellular experiment ，revealed that R-phycocyanin（40 μg/mL) could reduce the protein secretion of IL-4 and IL-13 in protein level.Gene expression analysis using lymphocytes showed that R -phycocyanin could reduce the expression of IL -4.In conclusion，R-phycocyanin could reduce food allergic reaction via skewing the immunological function of CD4+ T cell toward TH1 activity

参考文献/References:

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更新日期/Last Update: 2014-06-28