|本期目录/Table of Contents|

[1]张芳芳,熊静,段明珠,等.日本鳗鲡COX-2基因的克隆、鉴定及表达分析[J].集美大学学报(自然科学版),2015,20(5):321-332.
 ZHANG Fang-fang,XIONG Jing,DUAN Ming-zhu,et al.Molecular Cloning,Characterization and Expression of COX-2 in Japanese eel,Anguilla japonica[J].Journal of Jimei University,2015,20(5):321-332.
点击复制

日本鳗鲡COX-2基因的克隆、鉴定及表达分析(PDF)
分享到:

《集美大学学报(自然科学版)》[ISSN:1007-7405/CN:35-1186/N]

卷:
第20卷
期数:
2015年第5期
页码:
321-332
栏目:
水产、食品与生物工程
出版日期:
2015-09-30

文章信息/Info

Title:
Molecular Cloning,Characterization and Expression of COX-2 in Japanese eel,Anguilla japonica
作者:
张芳芳1熊静12段明珠1李春艳1彭喜霞1黄文树123
(1.集美大学水产学院,福建 厦门 361021;2.鳗鲡现代产业技术教育部工程研究中心,福建 厦门 361021;3.福建省海洋生物资源开发利用协同创新中心,福建 厦门 361021)
Author(s):
ZHANG Fang-fang1XIONG Jing12DUAN Ming-zhu1LI Chun-yan1PENG Xi-xia1HUANG Wen-shu123
(1.Fishery College,Jimei University,Xiamen 361021,China;2.Engineering Research Center ofthe Modern Technology for Eel Industry,Ministry of Education,Xiamen 361021,China;3.Fujian Collaborative Innovation Center for Exploitation and Vtiligation of Marine Biological Resources,Xiamen 361021,China)
关键词:
日本鳗鲡COX-2脂多糖多聚胞苷酸迟缓爱德华氏菌
Keywords:
Anguilla japonicaCOX-2LPSPoly I∶CEdwardsiella tarda
分类号:
-
DOI:
-
文献标志码:
A
摘要:
应用PCR及RACE技术从日本鳗鲡(Anguilla japonica)中克隆获得一个2463 bp环氧化酶基因(Cyclooxygenase-2,AjCOX-2)的cDNA序列.AjCOX-2前体为606肽,含有脊椎动物COX-2的保守结构域,以及加氧酶和过氧化物酶的活性位点.在正常生理条件下,AjCOX-2基因在日本鳗鲡的鳃、鳔和皮肤中高水平转录表达.脂多糖刺激后8 h,AjCOX-2基因在皮肤和鳔中的转录表达显著上调(P<0.05);多聚胞苷酸刺激后8 h,AjCOX-2在皮肤、鳔和鳃中的转录表达显著上调(P<0.05);迟缓爱德华氏菌(Edwardsiella tarda)刺激后8 h,AjCOX-2在鳔中的转录表达也极显著上调(P<0.01).结果表明,AjCOX-2基因是对外来病原刺激的早期响应基因,参与机体抵抗外来刺激物的先天免疫应答.
Abstract:
In this study,a full-length cDNA for Cyclooxygenase-2(AjCOX-2) was successfully cloned from the Japanese eel(Anguilla japonica) by PCR and RACE.The predicted protein of AjCOX-2 is 606 amino acids in length,which contained all the conservative structural domains and the active sites for both the peroxidase and cyclooxygenase as in its vertebrate homologues.In physiological condition,AjCOX-2 mRNA was highly expressed in gill,skin and swim bladder in Japanese eel.Additionally,a significant increasing AjCOX-2 mRNA was detected in both skin and swim bladder(P<0.05) at 8 h after in vivo intraperitoneal challenged with LPS and Poly I∶C,whilst a significant up-regulation of AjCOX-2 mRNA was found only in swim bladder at 8 h after challenged by Edwardsiella tarda,a pathogenic bacterium isolated from Japanese eel.The results suggested that AjCOX-2 might be an early immediate gene in response to the immune stimuli and involved in the innate immune response against the pathogens infection in Japanese eel.

参考文献/References:

-

相似文献/References:

[1]徐同玲,关瑞章,梁英,等.日本鳗鲡不同组织器官的抗菌活性比较[J].集美大学学报(自然科学版),2010,15(3):172.
 XU Tong-ling,GUAN Rui-zhang,LIANG Ying,et al.Antibacterial Activities in Different Tissues or Organs of Japanese Eel,Anguilla japonica[J].Journal of Jimei University,2010,15(5):172.
[2]李辉,钟毅雪,翁凌,等.日本鳗鲡胰蛋白酶的分离纯化及性质分析[J].集美大学学报(自然科学版),2016,21(2):99.
 LI Hui,ZHONG Yi-xue,WENG Ling,et al.Purification and Characterization of a Trypsin from the Hepatopancreas of Japanese Eel(Anguilla japonica)[J].Journal of Jimei University,2016,21(5):99.
[3]韩金钢,关瑞章,黄文树,等.日本鳗鲡肝肾病原菌及其防治中药的研究[J].集美大学学报(自然科学版),2011,16(5):327.
 HAN Jin-gangGUAN Rui-zhangHUANG Wen-shuJIN HengGUO Song-lin.16S rDNAInvestigations into the Pathogenic Bacteria from Liver and Kidney of Anguilla japonica and the Chinese Herbals for Controlling[J].Journal of Jimei University,2011,16(5):327.
[4]王佩,段明珠,黄贝,等.日本鳗鲡抗菌蛋白BPI基因的克隆、鉴定与表达[J].集美大学学报(自然科学版),2019,24(2):81.
 WANG Pei,DUAN Mingzhu,HUANG Bei,et al.Molecular Cloning and Expression Analysis of Two Genes Encoding Bactericidal/Permeability­Increasing Protein in Japanese Eel,Anguilla japonica[J].Journal of Jimei University,2019,24(5):81.
[5]何乐,翟少伟,冯建军,等.日本鳗鲡嗜水气单胞菌的分离鉴定与免疫相关基因变化的研究[J].集美大学学报(自然科学版),2019,24(4):249.
 HE Le,ZHAI Shaowei,FENG Jianjun,et al.Isolation and Identification of Pathogenic Aeromonas hydrophila from Japanese Eel (Anguilla japonica) and the Different Expression of Immune Related Genes After Eels Naturally Infected by A.hydrophila[J].Journal of Jimei University,2019,24(5):249.
[6]陈鹏云,林鹏,王艺磊,等.日本鳗鲡TRAF3基因的克隆、表达及亚细胞定位分析[J].集美大学学报(自然科学版),2023,28(3):193.
 CHEN Pengyun,LIN Peng,WANG Yilei,et al.Molecular Cloning,Expression Analysis in vivo and in vitro,and Subcellular Localization of TRAF3 from Japanese Eel (Anguilla japonica)[J].Journal of Jimei University,2023,28(5):193.
[7]赵歌洁,黄贝,黄文树.日本鳗鲡TBK-1基因的克隆及免疫刺激的表达模式[J].集美大学学报(自然科学版),2024,29(1):1.
 ZHAO Gejie,HUANG Bei,,et al.Cloning and Expression Pofile Analysis After Immune Stimulation of TBK-1 Gene in Japanese Eel (Anguilla japonica)[J].Journal of Jimei University,2024,29(5):1.

备注/Memo

备注/Memo:
-
更新日期/Last Update: 2015-11-03