|本期目录/Table of Contents|

[1]高贺,王新侠,倪辉,等.产微球茎菌琼胶酶基因的克隆及生物信息学分析[J].集美大学学报(自然科学版),2016,21(4):261-268.
　GAO He,WANG Xin-xia,NI Hui,et al.Cloning and Bioinformatics Analysis of the Agarase Gene from Microbulbifer sp.AG1[J].Journal of Jimei University,2016,21(4):261-268.
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《集美大学学报（自然科学版）》[ISSN:1007-7405/CN:35-1186/N]

卷:
第21卷
期数:
2016年第4期
页码:
261-268
栏目:
水产、食品与生物工程
出版日期:
2016-07-28

文章信息/Info

Title:
Cloning and Bioinformatics Analysis of the Agarase Gene from Microbulbifer sp.AG1
作者:
高贺1; 王新侠1; 倪辉1; 2; 3; 4; 肖安风1; 2; 3; 4; 蔡慧农1; 2; 3; 4; 朱艳冰1; 2; 3; 4
（1.集美大学食品与生物工程学院，福建 厦门 361021； 2.福建省食品微生物与酶工程重点实验室，福建 厦门361021； 3.厦门市食品与生物工程技术研究中心，福建 厦门 361021；4.厦门市南方海洋研究中心经济海藻资源化利用与深加工重点实验室，福建 厦门 361021）
Author(s):
GAO He1; WANG Xin-xia1; NI Hui1; 2; 3; 4; XIAO An-feng1; 2; 3; 4; CAI Hui-nong1; 2; 3; 4; ZHU Yanbing1; 2; 3; 4
(1.College of Food and Biological Engineering,Jimei University,Xiamen 361021,China;2.Fujian Provincial Key Laboratory of Food Microbiology and Enzyme Engineering,Xiamen 361021,China;3.Research Center of Food Biotechnology of Xiamen City,Xiamen 361021,China;4.Key Laboratory of Systemic Utilization and Indepth Processing of Economic Seaweed,Xiamen Southern Ocean Technology Center of China,Xiamen 361021,China)
关键词:
产微球茎菌; 琼胶酶; 克隆; 生物信息学
Keywords:
Microbulbifer sp.; agarase; cloning; bioinformatics
分类号:
-
DOI:
-
文献标志码:
A
摘要:
以琼脂为唯一碳源的培养基分离出一株产琼胶酶的海洋菌株AG1，16S rRNA基因序列分析显示，该菌株为产微球茎菌（Microbulbifer sp.）。以菌株AG1的基因组为模板，使用琼胶酶特异性引物进行PCR扩增，将扩增产物克隆至pMD18-T载体后进行测序。结果显示，克隆基因的大小为1302 bp，预测编码含有433个氨基酸残基的蛋白质。对该蛋白质进行生物信息学分析，结果表明，该蛋白质序列与来自耐热微泡菌（Microbulbifer thermotolerans）的琼胶酶氨基酸序列相似性为100%，预测本研究克隆的基因编码琼胶酶。该琼胶酶的理论分子质量大小为48.2 ku，理论等电点为5.42。采用同源建模法建立Microbulbifer sp.AG1琼胶酶的三维结构，富含β-折叠。
Abstract:
By using agar as the sole carbon source in the culture medium,a marine bacterium strain AG1 producing agarases was isolated.It was identified as Microbulbifer sp.AG1 based on 16S rRNA gene sequences alignment.The genomic DNA of this strain was used as the template for amplification of agarase gene by using PCR with a pair of specific primers.The amplified products were cloned into pMD18-T vector and then sequenced.It showed that the cloned gene was 1302 bp, encoding 433 amino acid residues.This protein was further analyzed by bioinformatics.The results showed that the target protein sequence shared 100% identity with the agarase sequence from Microbulbifer thermotolerans.The theoretical molecular weight and pI of the agarase were 48.2 ku and 5.42, respectively.The three-dimensional structure of Microbulbifer sp.AG1 agarase was constructed by homology modeling and presented β-strands rich structure.

参考文献/References:

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相似文献/References:

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　MA Rui-ping,ZHU Yan-bing,NI Hui,et al.Optimal Cultivation of a Marine Bacterium NTa for Agarase Production[J].Journal of Jimei University,2014,19(4):259.
[2]邵嫄,姚德恒,洪清林,等.海洋弧菌NTi产琼胶酶的发酵条件优化[J].集美大学学报(自然科学版),2019,24(1):20.
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备注/Memo

备注/Memo:
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更新日期/Last Update: 2016-09-24