|本期目录/Table of Contents|

[1]龚诗琦,王志勇,肖世俊,等.黄姑鱼转录组SSR的开发与验证[J].集美大学学报(自然科学版),2016,21(4):241-246.
　GONG Shi-qi,WANG Zhi-yong,XIAO Shi-jun,et al.Development and Verification of SSR Based on Transcriptome of Yellow Drum, Nibea albiflora[J].Journal of Jimei University,2016,21(4):241-246.
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《集美大学学报（自然科学版）》[ISSN:1007-7405/CN:35-1186/N]

卷:
第21卷
期数:
2016年第4期
页码:
241-246
栏目:
水产、食品与生物工程
出版日期:
2016-07-28

文章信息/Info

Title:
Development and Verification of SSR Based on Transcriptome of Yellow Drum, Nibea albiflora
作者:
龚诗琦1; 2; 王志勇1; 2; 肖世俊1; 2; 林爱强1; 2; 谢仰杰1; 2
(1.集美大学水产学院，福建 厦门 361021；2.农业部东海海水健康养殖重点实验室，福建 厦门 361021)
Author(s):
GONG Shi-qi1; 2; WANG Zhi-yong1; 2; XIAO Shi-jun1; 2; LIN Ai-qiang1; 2; XIE Yang-jie1; 2
(1.Fisheries College，Jimei University，Xiamen 361021，China;2.Key Laboratory of Healthy Mariculture for East China Sea，Ministry of Agriculture，Xiamen 361021,China)
关键词:
黄姑鱼; 微卫星; 标记; 转录组; 测序
Keywords:
yellow drum(Nibea albiflora); SSR; loci; transcriptome; sequeneing
分类号:
-
DOI:
-
文献标志码:
A
摘要:
利用MISA软件对黄姑鱼转录组测序数据进行分析，共检索到微卫星（simple sequence repeats,SSR）位点12 254个，其中单碱基、二碱基、三碱基重复分别约占42.70%、32.11%、23.44%，四碱基、五碱基、六碱基重复仅分别约占1.60%、0.09%、0.06%。随机选出80个二至六碱基重复来设计PCR引物，有38对引物通过扩增可获得目的条带。利用南海海区野生黄姑鱼样本对其中的18个具有多态性的SSR位点进行扩增效果验证和评价，结果显示：18个SSR的平均等位基因数为7.67，平均有效等位基因数为4.571，平均观测杂合度为0.3984，平均期望杂合度为0.7518，平均多态信息含量为0.6799，其中有15个位点表现出高多态性（多态信息含量>0.5）。与传统方法相比较，利用转录组测序数据开发微卫星标记更加省时、高效。
Abstract:
This paper identified 12 254 SSR loci from the transcriptome data of Nibea albiflora using MISA program.Among them,mononucleotides were the most frequent（42.70%),followed by dinucleotides（32.11%）and trinucleotides（23.44%),quadr-,penta- and hexa-nucleotides accounted for 1.60%,0.09% and 0.06%,respectively.Eighty loci were chosen randomly to design the primers,and 38 primer sets produced strong PCR products matching their expected sizes,and 18 of them were polymorphic.The characteristics of 18 loci were estimated by using 30 individuals of yellow drum from South China Sea.The mean number of alleles was 7.67,and the mean effective number of alleles across loci was 4.571.The observed and expected heterozygosity per locus ranged from 0.000 to 0.828 and 0.235 to 0.917,with an average of 0.398 4 and 0.751 8,respectively.The average of polymorphism information content（PIC）was 0.679 9,and 15 loci were highly informative（PIC>0.5).Compared with traditional approaches,this SSR isolation method is more time-saving and more effective.

参考文献/References:

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备注/Memo

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更新日期/Last Update: 2016-09-24