[1]谢枫才,张宾,孙继鹏.藻油中DHA-EE的HPLC快速定量方法的建立[J].集美大学学报(自然版),2018,23(3):187-194.
 XIE Fengcai,ZHANG Bin,SUN Jipeng.HPLC Method for Rapid Determination of DHA-EE in Algae Oil[J].Journal of Jimei University,2018,23(3):187-194.
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藻油中DHA-EE的HPLC快速定量方法的建立()
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《集美大学学报(自然版)》[ISSN:1007-7405/CN:35-1186/N]

卷:
第23卷
期数:
2018年第3期
页码:
187-194
栏目:
水产、食品与生物技术
出版日期:
2018-05-28

文章信息/Info

Title:
HPLC Method for Rapid Determination of DHA-EE in Algae Oil
作者:
谢枫才12张宾1孙继鹏2
(1.浙江海洋大学食品与医药学院,浙江 舟山 316022;2.国家海洋局第三海洋研究所,国家海洋局海洋生物资源综合利用工程技术研究中心,福建 厦门 361005)
Author(s):
XIE Fengcai12ZHANG Bin1SUN Jipeng2
(1.College of Food Science and Pharmacy,Zhejiang Ocean University,Zhoushan 316022,China;2.Third Institute of Oceanography,State Oceanic Administration,Engineering Research Center of Marine Biological Resource Comprehensive Utilization,Xiamen 361005,China)
关键词:
藻油二十二碳六烯酸乙酯高效液相色谱定量分析
Keywords:
algae oilDHA-EEHPLCquantitative analysis
摘要:
建立高效液相色谱法(high performance liquid chromatography,HPLC)快速测定藻油中二十二碳六烯酸乙酯(docosahexaenoic acid ethyl ester,DHA-EE)含量的方法。将藻油经乙酯化处理后,采用优化后DHA-EE分析参数:色谱柱ZORBAX SB-C8(4.6 mm×250 mm),流动相V(甲醇)∶V(水)=90∶10,柱温为30 ℃,流速为1.0 mL/min,紫外检测波长为204 nm,进样量为10 μL。测定结果显示,DHA-EE在0.008 1~1.92 μg 范围内呈良好线性关系,线性回归方程为:y=39.91 x+765.11(R2=0.999 2),平均回收率为102.42%(n=5,RSD=2.25%),精密度(n=10,RSD=0.32%)较好,DHA-EE最低定量限达8.05 ng。表明,该方法可用于藻油及其制品中DHA-EE的检测分析。
Abstract:
High performance liquid chromatography(HPLC)method was developed to quantify DHA-EE in marine microalgae oil quickly.The optimized quality parameters for DHA-EE analysis were as follows:chromatographic column,ZORBAX SB-C8(4.6 mm×250 mm);mobile phase,methanol versus water ( 90∶10);the column temperature 30 ℃;the flow velocity 1.0 mL/min;UV wavelength 204 nm;the injection volume 10 μL.It presented a good linear relationship within range of 0.008 1-1.92 μg for DHA-EE,and the linear regression equation was y=39.91 x+765.11(R2=0.999 2). The average recovery rate was 102.42% (n=5,RSD=2.25%) with good precision(n=10,RSD=0.32%),and the minimum quantify limit reached 8.05 ng.This optimized HPLC method could be used in the determination and analysis of algal oil and DHA-EE
更新日期/Last Update: 2018-07-13