[1]邵嫄,姚德恒,洪清林,等.海洋弧菌NTi产琼胶酶的发酵条件优化[J].集美大学学报(自然版),2019,24(1):20-30.
 SHAO Yuan,YAO Deheng,HONG Qinglin,et al.Process Optimization of Agarases Fermentation by Vibrio natriegens NTi[J].Journal of Jimei University,2019,24(1):20-30.
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海洋弧菌NTi产琼胶酶的发酵条件优化()
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《集美大学学报(自然版)》[ISSN:1007-7405/CN:35-1186/N]

卷:
第24卷
期数:
2019年第1期
页码:
20-30
栏目:
水产、食品与生物技术
出版日期:
2019-01-28

文章信息/Info

Title:
Process Optimization of Agarases Fermentation by Vibrio natriegens NTi
作者:
邵嫄1姚德恒1洪清林2嵇海峰2姜泽东13倪辉13肖安风134
(1.集美大学食品与生物工程学院,福建 厦门 361021;2.福建省海藻多糖企业工程技术研究中心,福建 漳州 363100;3.福建省海洋功能食品工程技术研究中心,福建 厦门 361021;4.厦门市海洋功能食品重点实验室,福建 厦门 361021)
Author(s):
SHAO Yuan1YAO Deheng1HONG Qinglin2JI Haifeng2JIANG Zedong13NI Hui13XIAO Anfeng134
(1.College of Food and Biological Engineering,Jimei University,Xiamen 361021,China;2.Algal Polysaccharide Enterprise Engineering Technology Research Center in Fujian Province,Zhangzhou 363100,China;3.Fujian Provincial Engineering Technology Research Center of Marine Functional Food,Xiamen 361021,China;4.Xiamen Key Laboratory of Marine Functional Food,Xiamen 361021,China)
关键词:
琼胶酶海洋弧菌NTi摇瓶发酵工艺优化响应面试验
Keywords:
agaraseVibrio natriegens NTishaking flask fermentationprocess optimization response surface methodology
文献标志码:
A
摘要:
以琼胶酶活力为主要指标,采用单因素与响应面相结合的方法,对降解琼胶的海洋弧菌NTi(Vibrio natriegens NTi)发酵产琼胶酶的摇瓶发酵培养基和发酵条件进行优化。结果表明,α-乳糖对菌株产酶具有较强的抑制作用,NH4+不利于菌株生长和产酶。培养基分别以3.3 g/L琼脂、6.33 g/L酵母膏为唯一碳源及氮源,添加20 g/L的NaCl ,发酵海洋弧菌V.natriegens NTi产琼胶酶的效果最佳。发酵过程中的pH值、接种量、装液量、温度、发酵时间最优值分别为6.5、2%、32 mL、27 ℃和24 h。优化后,该菌产琼胶酶活力达到2.81 U/mL,比优化前增加了230%。
Abstract:
The single factor test and response surface methodology were used to optimize the fermentation medium and fermentation conditions of agarase produced by marine Vibrio natriegens NTi,and the agarase activity was taken as main indicator.The results showed that α-lactose had a significant inhibitory effect on the agarase production,while ammonium ion was not conducive to cell growth and enzyme production.The optimal composition of culture medium for agarase-producing was:3.3 g/L agar as the sole carbon source,6.33 g/L yeast extract as the sole nitrogen source and 20 g/L NaCl.Meanwhile,the optimum conditions for agarase production were pH=6.5,inoculation amount 2%,liquid volume 32 mL,temperature 27 ℃ and fermentation time 24 h,respectively.Under the optimum conditions,agarase activity reached 2.81 U/mL after 24 h of fermentation and increased by 230% compared with the activity under the initial medium and conditions.

相似文献/References:

[1]马芮萍,朱艳冰,倪辉,等.海洋细菌NTa发酵产琼胶酶条件的初步优化[J].集美大学学报(自然版),2014,19(4):259.
 MA Rui-ping,ZHU Yan-bing,NI Hui,et al.Optimal Cultivation of a Marine Bacterium NTa for Agarase Production[J].Journal of Jimei University,2014,19(1):259.
[2]高贺,王新侠,倪辉,等.产微球茎菌琼胶酶基因的克隆及生物信息学分析[J].集美大学学报(自然版),2016,21(4):261.
 GAO He,WANG Xin-xia,NI Hui,et al.Cloning and Bioinformatics Analysis of the Agarase Gene from Microbulbifer sp.AG1[J].Journal of Jimei University,2016,21(1):261.

更新日期/Last Update: 2019-03-10