[1]焦超,李婧雅,李利君,等.固定化柚皮苷酶解转化制备普鲁宁[J].集美大学学报(自然版),2019,24(2):110-117.
 JIAO Chao,LI Jingya,LI Lijun,et al.Preparation of Pruning by Immobilized Naringin[J].Journal of Jimei University,2019,24(2):110-117.
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固定化柚皮苷酶解转化制备普鲁宁()
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《集美大学学报(自然版)》[ISSN:1007-7405/CN:35-1186/N]

卷:
第24卷
期数:
2019年第2期
页码:
110-117
栏目:
水产、食品与生物技术
出版日期:
2019-03-28

文章信息/Info

Title:
Preparation of Pruning by Immobilized Naringin
作者:
焦超1李婧雅1李利君12杨秋明12 翁惠芬13肖安风123
(1.集美大学食品与生物工程学院,福建 厦门 361021;2.福建省食品微生物与酶工程重点实验室,福建 厦门 361021;3.福建省海洋功能食品工程技术研究中心,福建 厦门 361021)
Author(s):
JIAO Chao1LI Jingya1LI Lijun12YANG Qiuming12WENG Huifen13XIAO Anfeng123
(1.College of Food and Biological Engineering,Jimei University,Xiamen 361021,China;2.Key Laboratory of Food Microbiology and Enzyme Engineering of Fujian Province,Xiamen 361021,China;3.Fujian Provincial Engineering Technology Research Center of Marine Functional Food,Xiamen 361021,China)
关键词:
普鲁宁底物固定化柚皮苷酶法转化
Keywords:
pruningsubstrate immobilizationnaringinenzymatic transformation
文献标志码:
A
摘要:
选择D101-1大孔吸附树脂固定化反应底物柚皮苷,考察α-L-鼠李糖苷酶水解柚皮苷转化普鲁宁的工艺条件,结果表明,其最适条件为:α-L-鼠李糖苷酶用量30 U/mL,最适酶反应温度60 ℃,pH=4.0,底物质量浓度2.4 g/L,振荡速率80 r/min。在此条件下酶解反应420 min,柚皮苷转化率达到78%。此外,研究发现,高浓度的Mn2+、Fe2+对酶解反应有极强的促进作用。Lineweaver-Burk双倒数拟合曲线的Km=5.12 g/L,Vmax=0.013 g/(L·min)。利用薄层色谱(thin layer chromatography,TLC)和高效液相色谱(high performance liquid chromatography,HPLC)对酶解产物进行分析,并验证得出,反应完全后可得到纯的反应产物普鲁宁。
Abstract:
Naringin immobilized with D101-1 macroporous resin was used as the reaction substrate,the process conditions of α-L-rhamnosidase hydrolysis of naringin to convert pruning were investigated.The conditions for pruning transformation were optimized as α-L-rhamnosidase dosage 30 U / mL,reaction temperature 60 ℃,pH=4.0,substrate concentration 2.4 g/L,shaking rate 80 r/min.Under the optimal conditions,the conversion of naringin reached 78% after enzymatic hydrolysis for 420 min.In addition,it was found that high concentration of Mn2+,Fe2+ could significantly promote the enzymatic reaction.The Km and Vmax of Lineweaver-Burk fitted curve were 5.12 g/L and 0.013 g/(L·min),respectively.The analysis of the hydrolyzate by TLC and HPLC showed that pure pruning could be obtained after the reaction was completed.
更新日期/Last Update: 2019-05-05