|本期目录/Table of Contents|

[1]鲍俊旺,李越,翁凌,等.凡纳滨对虾多酚氧化酶的纯化及性质分析[J].集美大学学报(自然科学版),2019,24(2):100-109.
　BAO Junwang,LI Yue,WENG Ling,et al.Purification and Characterization of Polyphenol Oxidase from Pacific White Shrimp Litopenaeus vannamei[J].Journal of Jimei University,2019,24(2):100-109.
点击复制

凡纳滨对虾多酚氧化酶的纯化及性质分析(PDF)
分享到：

《集美大学学报（自然科学版）》[ISSN:1007-7405/CN:35-1186/N]

卷:
第24卷
期数:
2019年第2期
页码:
100-109
栏目:
水产、食品与生物工程
出版日期:
2019-03-28

文章信息/Info

Title:
Purification and Characterization of Polyphenol Oxidase from Pacific White Shrimp Litopenaeus vannamei
作者:
鲍俊旺1; 李越1; 翁凌1; 2; 张凌晶1; 2; 孙乐常1; 2; 曹敏杰1; 2
( 1.集美大学食品与生物工程学院，福建 厦门 361021；2.水产品深加工技术国家地方联合工程研究中心，福建 厦门 361021)
Author(s):
BAO Junwang1; LI Yue1; WENG Ling1; 2; ZHANG Lingjing1; 2; SUN Lechang1; 2; CAO Minjie1; 2
(1.College of Food and Biological Engineering,Jimei University,Xiamen 361021,China；2.National & Local Joint Engineering Research Center for Aquatic Products Processing,Xiamen 361021,China)
关键词:
凡纳滨对虾; 多酚氧化酶; 纯化; 黑变
Keywords:
Litopenaeus vannamei; polyphenol oxidase; purification; melanosis
分类号:
-
DOI:
-
文献标志码:
A
摘要:
通过硫酸铵盐析、DEAE-Cellulose离子交换、Phenyl-Sepharose疏水层析、Hi-Trap Capto-Q强阴离子交换层析等方法，从凡纳滨对虾（Litopenaeus vannamei）虾头中分离纯化出一种多酚氧化酶（polyphenol oxidase，PPO），十二烷基硫酸钠-聚丙烯酰胺凝胶电泳（sodium dodecyl sulfate polyacrylamide gel electropheresis，SDS-PAGE）显示其分子质量约为210 ku。肽质量指纹图谱的比对结果表明，纯化得到的PPO中含有187个氨基酸残基的14个片段与NCBI数据库中酚氧化酶原（gi|147724160）序列相似性为100%。该酶的最适温度和最适pH值分别为40 ℃和6.0，且在温度0~50 ℃及pH=5.0~8.0可保持相对稳定的活性。圆二色谱分析结果表明，对虾PPO的二级结构主要为反平行结构及无规则卷曲。抗坏血酸、茶多酚和特丁基对苯二酚(tertiary butylhydroquinone，TBHQ)等具有生物活性羟基的抑制剂对其均有较好的抑制效果。
Abstract:
In this study,a polyphenol oxidase (PPO) was purified to homogeneity from Pacific white shrimp (Litopenaeus vannamei) by ammonium sulfate fractionation and column chromatographies including DEAE-Cellulose,Phenyl-Sepharose HP and Hi-Trap Capto-Q. SDS-PAGE showed that the molecular weight of L.vannamei PPO was about 210 ku and its optimal temperature and pH were 40 ℃ and 6.0,respectively.Comparison of sequences between the purified PPO and prophenol oxidase (gi|147724160) of L.vannamei in the database of NCBI showed a similarity of 100% on 187 amino acid residues of 14 peptide fragments.The activity of PPO remained stable at 0～50 ℃ and in the pH range of 5.0～8.0.The secondary structure of PPO was mainly anti-parallel β-sheet and random coil as revealed by circular dichrosim(CD).Additives of ascorbic acid,tea-polyphenols,and tertiary butylhydroquinone (TBHQ) inhibited the enzyme activity effectively.

参考文献/References:

相似文献/References:

[1]王玲,张春晓,钟幼平.对虾、东方鲀和星虫混养模式的氮磷收支研究[J].集美大学学报(自然科学版),2013,18(3):172.
　WANG Ling,ZHANG Chun-xiao,ZHONG You-ping.An Experimental Study on Nitrogen and Phosphorus Budgets in Closed Polyculture of Litopenaeus vannamei Takifugu flavidus and Phascolosoma esculenta[J].Journal of Jimei University,2013,18(2):172.
[2]黄飞,张春晓,王玲,等.矿物元素平衡对鸡肉粉替代凡纳滨对虾饲料鱼粉的影响[J].集美大学学报(自然科学版),2016,21(1):1.
　HUANG Fei,ZHANG Chun-xiao,WANG Ling,et al.Effects of Mineral Balance on Fish Meal Replacement with Poultry by Product Meal in Diets for Litopenaeus vannamei[J].Journal of Jimei University,2016,21(2):1.
[3]胡文燕,朱孟凯,钟理,等.凡纳滨对虾T7噬菌体cDNA展示文库的构建[J].集美大学学报(自然科学版),2017,22(2):11.
　HU Wenyan,ZHU Mengkai,ZHONG Li,et al.Construction of T7 Phage Display cDNA Library of Litopenaeus vannamei[J].Journal of Jimei University,2017,22(2):11.
[4]胡水城,鲁康乐,宋凯,等.复合蛋白CM2替代鱼粉对凡纳滨对虾生长、表观消化率和抗氧化能力的影响[J].集美大学学报(自然科学版),2017,22(5):10.
　HU Shuicheng,LU Kangle,SONG Kai,et al.Effects of Fishmeal Replacement by Compound Protein(CM2) on Growth, Apparent Digestibility and Antioxidant of Litopenaeus vannamei[J].Journal of Jimei University,2017,22(2):10.

备注/Memo

备注/Memo:
更新日期/Last Update: 2019-05-05