[1]何乐,翟少伟,冯建军,等.日本鳗鲡嗜水气单胞菌的分离鉴定与免疫相关基因变化的研究[J].集美大学学报(自然版),2019,24(4):249-257.
 HE Le,ZHAI Shaowei,FENG Jianjun,et al.Isolation and Identification of Pathogenic Aeromonas hydrophila from Japanese Eel (Anguilla japonica) and the Different Expression of Immune Related Genes After Eels Naturally Infected by A.hydrophila[J].Journal of Jimei University,2019,24(4):249-257.
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日本鳗鲡嗜水气单胞菌的分离鉴定与免疫相关基因变化的研究()
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《集美大学学报(自然版)》[ISSN:1007-7405/CN:35-1186/N]

卷:
第24卷
期数:
2019年第4期
页码:
249-257
栏目:
水产、食品与生物技术
出版日期:
2019-07-28

文章信息/Info

Title:
Isolation and Identification of Pathogenic Aeromonas hydrophila from Japanese Eel (Anguilla japonica) and the Different Expression of Immune Related Genes After Eels Naturally Infected by A.hydrophila
作者:
何乐12翟少伟12冯建军12江兴龙12肖益群12郭松林12
(1.集美大学水产学院,福建 厦门 361021;2.鳗鲡现代产业技术教育部工程研究中心,福建 厦门 361021)
Author(s):
HE Le12ZHAI Shaowei12FENG Jianjun12JIANG Xinglong12XIAO Yiqun12GUO Songlin12
(1.Fisheries Colleage,Jimei University,Xiamen 361021,China;2.Engineering Research Center of the Modern Industry Technology for Eel,Ministry of Education,Jimei University,Xiamen 361021,China)
关键词:
日本鳗鲡嗜水气单胞菌自然感染免疫相关基因
Keywords:
Japanese eelAeromonas hydrophilanatural infectionimmune-related gene
文献标志码:
A
摘要:
2018年1—2月,饲养于集美大学疫苗中心的日本鳗鲡(Anguilla japonica)出现了一定数量的死亡,从病鱼脾脏中分离到一株优势菌株,结合Biolog系统微生物鉴定和分离菌16S rRNA基因的测序结果,显示该分离菌为嗜水气单胞菌(Aeromonas hydrophila)。采集自然感染嗜水气单胞菌前后的日本鳗鲡肝、脾、肾和粘液为cDNA模板,采用荧光定量PCR(real-time PCR)法检测组织中Toll样受体3(tlr3)、肿瘤坏死因子(tnf-α)、C型溶菌酶(lysC)和环氧合酶(cox2)等免疫相关基因的表达变化。结果表明:日本鳗鲡自然感染嗜水气单胞菌后,tlr3、tnf-α、cox2均未发现显著性的变化,但lysC的含量在日本鳗鲡脾脏和肾脏中均出现显著性上调,即,自然感染嗜水气单胞菌的日本鳗鲡的抗感染免疫功能与C型溶菌酶密切相关。
Abstract:
From January to February in 2018,some Japanese eel farmed in the vaccine center of Jimei University (Fujian Province,China)at 25 ℃ was infected by bacterial pathogensand showed a certain number of deaths.A dominant bacteria strain was isolated from the spleen of the diseased eels.By Biolog identification and 16S rRNA gene sequence analysis,the strain was identified as Aeromonas hydrophila.Then,RNA from kidney,spleen,liver and mucus of 5 diseased and 5 uninfected eels were extracted to detect the expressions of tnf-α,lysC,cox2 and tlr3 genes by quantitative real-time PCR.The results showed that the expression of lysC was significantly upregulated between the infected and uninfected eels in spleen and kidney,but no significant difference was found in tnf-α,cox2 and tlr3 genes.The result indicated that the C-type lysozyme was closely related to the anti-infection immune function of Japanese eels naturally infected by A.hydrophila.

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更新日期/Last Update: 2019-08-31