[1]朱阳阳,张梦,叶坤,等.17β-雌二醇对大黄鱼性别分化相关基因表达的影响[J].集美大学学报(自然版),2019,24(6):401-408.
 ZHU Yangyang,ZHANG Meng,YE Kun,et al.Effects of 17-estradiol on the Expression of Genes Related to Sex Differentiation of the Large Yellow Croaker[J].Journal of Jimei University,2019,24(6):401-408.
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17β-雌二醇对大黄鱼性别分化相关基因表达的影响()
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《集美大学学报(自然版)》[ISSN:1007-7405/CN:35-1186/N]

卷:
第24卷
期数:
2019年第6期
页码:
401-408
栏目:
水产、食品与生物技术
出版日期:
2019-11-28

文章信息/Info

Title:
Effects of 17-estradiol on the Expression of Genes Related to Sex Differentiation of the Large Yellow Croaker
作者:
朱阳阳12张梦12叶坤12沈伟良3吴雄飞3王志勇12
(1.集美大学水产学院,福建 厦门 361021;2. 农业部东海海水健康养殖重点实验室,福建 厦门 361021 ;3.宁波市海洋与渔业研究院,浙江 宁波 315103 )
Author(s):
ZHU Yangyang12ZHANG Meng12YE Kun12SHEN Weiliang3WU Xiongfei3WANG Zhiyong12
(1.Fisheries College,Jimei University,Xiamen 361021,China;2.Key Laboratory of Healthy Mariculture for the East China SeaMinistry of AgricultureJimei UniversityXiamen 361021China;3.Ningbo Institute of Ocean and Fishery,Ningbo 315103,China)
关键词:
大黄鱼雌二醇性别分化基因表达
Keywords:
large yellow croaker(Larimichthys crocea)estradiolsex differentiationgene expression
摘要:
为了解17β-雌二醇(E2)对大黄鱼性别决定与性腺发育相关基因表达的影响,在鱼苗培育到41 dph(days post-hatch)时分别投喂添加了0、80、120 mg/kg 17β-雌二醇的配合饲料,并在开始饲喂前(41 dph)和开始饲喂后第30天(70 dph)、第50天(90 dph)、第80天(120 dph)取样,用大黄鱼性别特异分子标记进行遗传性别鉴定后,挑选出遗传雄性(XY)个体,采用qRT-PCR技术检测性腺中amh、gsdf、cyp19a和foxl2四个性别发育与分化相关基因的表达情况。结果表明,E2能下调amh、gsdf、foxl2的表达,对不同发育阶段的大黄鱼体内的性激素转化通路(cyp19a)的影响不同。
Abstract:
The goal of the present study was to investigate the effect of 17β-estradiol(E2) treatment on the expression of genes related to sex determination and gonadal development in the large yellow croaker.When the fry were grown to 41dph(days post-hatch),they were fed with compound feed containing 0,80 and 120 mg/kg 17β-estradiol,and sampled before starting feeding (41 dph) and 30th d (70 dph),50th d (90 dph),80th d (120 dph) after starting feeding.After genetic sex identification with sex-specific molecular markers of the large yellow croaker genetic male (XY) individuals were selected,quantitative real-time PCR(qRT-PCR)was used to analyze the expression levels of genes involved in sexual development and differentiation including amh,gsdf,cyp19a and foxl2 in gonads.The results indicated that E2 could down-regulate the expression of amh,gsdf and foxl2,and has different effects on the sex hormone conversion pathway (cyp19a) in the large yellow croakers at different developmental stages.

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更新日期/Last Update: 2020-01-01