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[1]刘璐,刘祥梅,谭军,等.小鼠心肌MHC和TM1基因表达的运动训练实验研究[J].体育科学研究,2015,19(06):47-52.
　LIU Lu,LIU Xiang-mei,TAN Jun,et al.The Leek Hawthorn Loach Soup Effect on Swimming Training and Exhaustion Mice Myocardial MHC and TM1 Gene Expression[J].sports science research,2015,19(06):47-52.
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《体育科学研究》[ISSN:1006-6977/CN:61-1281/TN]

卷:
19
期数:
2015年06
页码:
47-52
栏目:
体育生物科学
出版日期:
2015-12-31

文章信息/Info

Title:
The Leek Hawthorn Loach Soup Effect on Swimming Training and Exhaustion Mice Myocardial MHC and TM1 Gene Expression
作者:
刘璐; 刘祥梅; 谭军; 施孟
(湖南师范大学体育学院,湖南 长沙 410012）
Author(s):
LIU Lu; LIU Xiang-mei; TAN Jun; SHI Meng
(College of Physical Education Science, Hunan Normal University, Changsha 410012, China)
关键词:
韭楂泥鳅; 力竭运动; 心肌; MHC基因; TM1基因
Keywords:
leek hawthorn loach soup; exhaustion exercise; myocardium; MHC mRNA; TM1 mRNA
分类号:
-
DOI:
-
文献标志码:
A
摘要:
从对心肌MHC和TM1基因表达的影响，探讨韭楂泥鳅汤提高小鼠运动能力的作用及机制。将健康雄性ICR小鼠32只，按体重分层随机分为安静对照组(C)、运动训练组(T)、泥鳅运动组1(N1)、泥鳅运动组2(N2)，每组8只。T组和N组游泳训练三周后，尾根部负重5 %进行力竭游泳，记录游泳至力竭时间。24 h后取材，检测心系数，HE染色观察心肌组织结构，RT-PCR测定心肌组织α-MHC、β-MHC和TM1的基因表达。表明：与T组比较，小鼠的力竭运动时间N1组显著延长(P<0.05)，N2组呈延长趋势但无显著性差异(P＞0.05)。与C组比较，T组小鼠的心系数显著增高(P<0.05)，N1组呈增高趋势而无显著性差异(P＞0.05)；光镜下，C组心肌细胞界限清晰、结构正常；力竭后24 h，T组的心肌细胞界限模糊，可见心肌纤维断裂和间质水肿，而N1组接近C组；与C组比较，T组的β-MHC mRNA表达显著增高(P<0.05)，α/β-MHC mRNA比值显著降低(P<0.05)；N1组的α-MHC mRNA、β-MHC mRNA 和TM1 mRNA表达较C组和T组均增高(P<0.05)，但α/β-MHC mRNA比值与C组差异不显著(P＞0.05)。合适应用韭楂泥鳅汤能延长小鼠力竭游泳时间，具有抗运动疲劳作用，与其稳定肌球蛋白重链基因表达比和增强原肌球蛋白1基因的表达相关。
Abstract:
(Objective) By analyzing influences on myocardial MHC and TM1 gene expression, the experiment has discussed the function and mechanism of the leek hawthorn loach soup that improves mices movement capacity.(Methods) 32 healthy male ICR mice are randomly divided into sedentary control group (C), exercise-trained group (T) , Loach movement group 1(N1) and Loach movement group 2 (N2)(n=8 animals/group) on the basis of weight. After three weeks of swim training on group T and group N, these mice start to swim exhaustively with the tail root 5 percent weight. The exhaustive swimming time was record. After 24 hours, coefficient of the heart was detected, cardiac muscle tissue structure was observed by microscope after HE staining, Expressions of myocardial α-MHC and β-MHC mRNA were detected by RT-PCR.(Results) 1.N1 group was significantly longer than T group(P＜0.05) on the time of exercise exhaustive. Compared with C group heart coefficient increased significantly (P＜0.05) in T group mice.2.The myocardial cell of C group was clear and normal structure; myocardial cell was fuzzy, visible myocardial fiber fracture and interstitial edema in T group; while N1 group is close to C group.3.Compared with group C β-MHC mRNA expression is significantly higher(P＜0.05),α/β-MHC mRNA decreases significantly（P＜0.05）in T group；N1 group of α-MHC mRNA,β-MHC mRNA and TM1 mRNA expression are higher than C group and T group(P＜0.05)，but the α/β-MHC mRNA expression has no significant difference with C group(P＞0.05).(Conclusion) The leek hawthorn loach soup can prolong exhaustive exercise time of mice by enhancing the resistance fatigue ability. This is related to protect the myocardial cell，stable proportion of myosin heavy chain gene expression and enhance TM1 gene expression.

参考文献/References:

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相似文献/References:

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备注/Memo

备注/Memo:
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更新日期/Last Update: 2015-12-29