«上一篇/Previous Article|本期目录/Table of Contents|下一篇/Next Article»

《集美大学学报（自然科学版）》[ISSN:1007-7405/CN:35-1186/N]

卷:

第15卷

期数:

2010年第2期

页码:

93-97

栏目:

水产科学与生物工程

出版日期:

2010-03-25

文章信息/Info

Title:

Influences of Blood and Biochemical Parameters on American Eels(Anguilla rostrata)Challenged by Aeromonas hydrophila

作者:

郭松林1; 2; 关瑞章1; 2; 冯建军1; 2; 陈传卿1

(1.集美大学水产学院,福建 厦门 361021;2.福建省高校水产科学与食品安全重点实验室,福建 厦门 361021)

Author(s):

GUO Song-lin1; 2; GUAN Rui-zhang1; 2; FENG Jian-jun1; 2; CHEN Chuan-qing1

(1.Fisheries College,Jimei University,Xiamen 361021,China;2.Key Laboratory of Science and Technology forAquaculture and Food Safety(Jimei University),Fujian Province,Xiamen 361021,China)

关键词:

美洲鳗鲡; 嗜水气单胞菌; 人工感染; 血液生化指标

Keywords:

American eels(Anguilla rostrata); Aeromonas hydrophila; artificial infection; blood biochemistry parameter

分类号:

-

DOI:

-

文献标志码:

-

摘要:

］为研究自发病美洲鳗鲡分离的嗜水气单胞菌对该鱼的致病性，试验将60尾美洲鳗鲡平均分为对照组、浸泡组和注射组3组，3组鳗鲡分别注射PBS、以1.0×107 cfu/mL的嗜水气单胞菌浸泡和1.0×108 cfu/mL嗜水气单胞菌注射感染.各组鳗鲡分别于处理后6 h和30 h断尾采血并分离抗凝血和血清，抗凝血由血常规检测分析仪检测7项血液指标，血清由全自动生化分析仪检测13项与免疫相关的生化指标，数据用SAS软件进行统计学分析.结果表明，鳗鲡注射感染后6 h，与浸泡组相比，血清谷草转氨酶(AST)与谷丙转氨酶(ALT)的比值显著（P<0.05）升高，其他指标在三组间均无显著（P>0.05）差异.鳗鲡注射感染后 30 h，同对照组和浸泡组相比，血清总蛋白和血糖极显著（P<0.01）下降，Na+和Cl-浓度显著（P<0.05）下降，而AST和ALT则均极显著（P<0.01）上升

Abstract:

To study the influences of blood and biochemical parameters on American eels(Anguilla rostrata)challenged by Aeromonas hydrophila,sixty American eels were randomly divided into PBS injection group(control group),bacteria immerging group treated with 1.0?07 cfu/mL live bacteria for one hour and bacteria injection group treated with 1.0?08 cfu/ mL live bacteria.Blood samples were taken from eel caudal sinus.Anti-coagulate blood samples and serum samples were used to determine the blood samples and wibiochemical parameters respectively by automatic biochemical apparatus(BECKMAN CX9).Obtained data were analyzed by SAS(Statistical Analysis System).The results showed that the ratio of aspartate aminotransferase (AST)and alanine aminotransferase(ALT)were significantly higher in injection group than in immersion group(P<0.05),and no significant differences were observed in other parameters among three groups(P>0.05).Serum total protein and blood sugar greatly declined(P<0.01),and concentration of Na+ and Cl- were significantly decreased(P<0.05),while AST and ALT distinctively(P<0.01)increased in injection group than in immersion group and control one after being challenged to 30 h

参考文献/References:

-

相似文献/References:

[1]王雷,李元跃,陈融斌,等.运用SELEX技术筛选嗜水气单胞菌适配子[J].集美大学学报(自然科学版),2010,15(6):1.
　WANG LeiLI Yuan-yueCHEN Rong-binLI Zhong-bao.Screening of the DNA Aptamers Against Aeromonas hydrophilaby Using SELEX Method[J].Journal of Jimei University,2010,15(2):1.
[2]张子华,鄢庆枇,邹文政,等.鳗鲡肠道中嗜水气单胞菌的拮抗菌筛选[J].集美大学学报(自然科学版),2010,15(3):165.
　Fisheries College,Jimei University,Xiamen 0,et al.Isolation and Screening of Antagonistic Bacteria Against Aeromonas hydrophila from Intestine of Anguilla anguilla ZHANG Zi-hua1,YAN Qing-pi1,2,ZOU Wen-zheng1,2,XING Yan-li1,2,GUO Song-lin1,2, JI Rong-xing1,2[J].Journal of Jimei University,2010,15(2):165.
[3]黄利强,许昱,郭松林.纳米TiO2光催化杀灭水产病原菌的研究[J].集美大学学报(自然科学版),2010,15(4):254.
　HUANG Li-qiang,XU YuGUO Song-lin.Disinfecting Aquatic Pathogenic Bacteria byPhotocatalytic Activity with Nanometer TiO2[J].Journal of Jimei University,2010,15(2):254.
[4]王雷,李元跃,陈融斌,等.运用SELEX技术筛选嗜水气单胞菌适配子[J].集美大学学报(自然科学版),2012,17(1):1.
[5]郭松林,王玉,冯建军,等.鳗鲡病原菌二联外膜蛋白基因工程表达载体的构建[J].集美大学学报(自然科学版),2014,19(5):330.
　GUO Song-lin,WANG Yu,FENG Jian-Jun,et al.Constrction of a Bivalent Expression Vector of Outer Membrane Protein of Two Pathogens Pathogenic to Eels[J].Journal of Jimei University,2014,19(2):330.
[6]许晓芸,陈锦民,冯建军,等.迟缓爱德华氏菌感染美洲鳗鲡后的组织与超微病理观察[J].集美大学学报(自然科学版),2017,22(6):17.
　XU Xiaoyun,CHEN Jinmin,FENG Jianjun,et al.Pathological and Ultrastructural Changes of American Eel（Anguilla rostrata）Infected by Edwardsiella tarda[J].Journal of Jimei University,2017,22(2):17.
[7]何乐,翟少伟,冯建军,等.日本鳗鲡嗜水气单胞菌的分离鉴定与免疫相关基因变化的研究[J].集美大学学报(自然科学版),2019,24(4):249.
　HE Le,ZHAI Shaowei,FENG Jianjun,et al.Isolation and Identification of Pathogenic Aeromonas hydrophila from Japanese Eel (Anguilla japonica) and the Different Expression of Immune Related Genes After Eels Naturally Infected by A.hydrophila[J].Journal of Jimei University,2019,24(2):249.
[8]肖益群,翟少伟,郭松林.鳗鲡源创伤弧菌的间接ELISA快速检测[J].集美大学学报(自然科学版),2020,25(2):81.
　XIAO Yiqun,ZHAI Shaowei,GUO Songlin.The Indirect ELISA for Rapid Detection of Vibrio vulnificus from Eels[J].Journal of Jimei University,2020,25(2):81.
[9]张世佳,冯建军,郭松林,等.超分支滚环扩增法检测美洲鳗鲡嗜水气单胞菌[J].集美大学学报(自然科学版),2021,26(3):193.
　ZHANG Shijia,FENG Jianjun,GUO Songlin,et al.Hyper-Branched Rolling Circle Amplification Assay for Detecting Aeromonas hydrophila in Anguilla rostrata[J].Journal of Jimei University,2021,26(2):193.
[10]王岳,翟少伟,万绮娟,等.鳗鲡爱德华氏菌对美洲鳗鲡的致病性与宿主抗感染相关基因的表达[J].集美大学学报(自然科学版),2024,29(2):97.
　WANG Yue,ZHAI Shaowei,WAN Qijuan,et al.Pathogenicity of Edwardsiella anguillarum to American Eels(Anguilla rostrota) and the Expression of Host Anti-E.anguillarum Infection Related Genes[J].Journal of Jimei University,2024,29(2):97.
[11]陈锦民,许晓芸,郭松林.嗜水气单胞菌感染美洲鳗鲡的病理观察[J].集美大学学报(自然科学版),2015,20(3):161.
　CHEN Jin-min,XU Xiao?yun,GUO Song?lin.Pathological Changes of American Eel(Anguilla rostrata) Infected by Aeromonas hydrophila[J].Journal of Jimei University,2015,20(2):161.

备注/Memo

备注/Memo:

-

常用功能

更新日期/Last Update: 2014-06-28