|本期目录/Table of Contents|

[1]徐同玲,关瑞章,梁英,等.日本鳗鲡不同组织器官的抗菌活性比较[J].集美大学学报(自然科学版),2010,15(3):172-178.
　XU Tong-ling,GUAN Rui-zhang,LIANG Ying,et al.Antibacterial Activities in Different Tissues or Organs of Japanese Eel,Anguilla japonica[J].Journal of Jimei University,2010,15(3):172-178.
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《集美大学学报（自然科学版）》[ISSN:1007-7405/CN:35-1186/N]

卷:
第15卷
期数:
2010年第3期
页码:
172-178
栏目:
水产科学与生物工程
出版日期:
2010-05-25

文章信息/Info

Title:
Antibacterial Activities in Different Tissues or Organs of Japanese Eel,Anguilla japonica
作者:
徐同玲1; 关瑞章1; 2; 梁英1; 2; 黄文树1; 2
(1. 集美大学水产学院,福建 厦门 361021;2. 福建省高校水产科学技术与食品安全重点实验室,福建 厦门 361021)
Author(s):
XU Tong-ling1; GUAN Rui-zhang1; 2; LIANG Ying1; 2; HUANG Wen-shu1; 2
(1.Fisheries College,Jimei University,Xiamen 361021,China;2.Key Laboratory of Science and Technology for Aquaculture and Food Safety(Jimei University),Fujian Province,Xiamen 361021,China)
关键词:
日本鳗鲡; 抗菌活性; 反相高效液相色谱
Keywords:
Anguilla japonica; antibacterial activity; reversed-phase high-performance liquid chromatography
分类号:
-
DOI:
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文献标志码:
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摘要:
］用体积分数为10 %的醋酸提取日本鳗鲡肝脏、鳃、脾脏、胆汁、肾脏和血清中的抗菌活性物质，检测其对养殖鳗鲡常见的4种致病菌，即肠杆菌Enterobacter sp.（B01）、迟缓爱德华氏菌Edwardsiella tarda(B09)、气单胞菌属Aeromona sp.(B18)和嗜水气单胞菌Aeromona hydrophila(B27)的抑杀作用.结果显示：1）肝脏、脾脏、胆汁和鳃的样品对4株试验菌株均有明显的抑菌作用，肾脏的对B18、B27和B01 3株致病菌有抑菌作用，血清的仅对B18有抑菌作用.2)不同样品对同一种致病菌的抗菌活性差异显著（P<0.05）,按平均抑菌圈半径计算，其抗菌活性大体呈现为：肝脏>鳃>脾脏>胆汁>肾脏>血清；同一样品对不同细菌的抑杀作用差异显著（P<0.05）.采用反相高效液相色谱法分析上述各组织/器官的醋酸提取物中的活性组分，结果显示：肝脏、脾脏、胆汁和鳃样品中的活性组分（以杀伤指数大于50 %计）主要集中于乙腈洗脱质量分数为19.4 %~35.4 %时，肾脏和血清中没有明显的抗菌作用（杀伤指数小于50 %）
Abstract:
Compared and analysed of the antibacterial activities in different tissues and organs,including liver,gill,spleen,bile,kidney and serum of A.japonica.This study obtained acid extractions of the tissues and organs from Japanese eels.Using agar diffusion method,the antibacterial activity of those extractions against four common pathogenic bacteria (Enterobacter spp.B01,Edwardsiella tarda B09,Aeromonas spp.B18 and A.hydrophila B27) from the cultivated eels was detected.The results showed that antibacterial activities of liver,gill,spleen,bile were obvious.The extractions of kidney exhibited antibacterial activity against B18,B27 and B01.The extractions of serum exhibited antibacterial activity against B18.The antibacterial activities of extractions from different tissues and organs were different to the same bacterium.The antibacterial activity of liver was the highest to all experimented bacteria.The antibacterial activity of extractions from the same tissues or organs varied to different bacteria.The acid extractions were further purified by the method of reverse-phase liquid chromatography on Source 5R RPC-ST-4.6/150 column （Amersham Bioscience,Sweden）.The results showed that the killing indexes of the extractions from liver,kidney,gill and bile were higher than 50 % when eluted by the concentration of aceonitrile ranging from 19.4 % to 35.4 %,while the killing index of extractions from serum and kidney were lower than 50 % when eluted by the concentration of aceonitrile ranging from 0 % to 80 %

参考文献/References:

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更新日期/Last Update: 2014-06-28