响应面法优化龙眼核多酚提取工艺的研究-《集美大学学报（自然科学版）》

文章信息/Info

Title:: Study on Extraction of Polyphenols from Longan Seed byResponse Surface Analysis

作者:: 吴兰兰1; 2; 汤凤霞1; 2; 何传波1; 2; 魏好程1; 2; (1.集美大学生物工程学院,福建厦门 361021;2.集美大学食品与生物工程研究所,福建厦门 361021)

Author(s):: WU Lan-lan12; TANG Feng-xia12; HE Chuan-bo12; WEI Hao-cheng12; (1.School of Biotechnology Engineering,Jimei University,Xiamen 361021,China;2.Institute of Food and Biotechnology Engineering,Jimei University,Xiamen 361021,China)

摘要:: ］以龙眼核为原料，用乙醇溶液为溶剂提取其中的酚类化合物，在单因素实验基础上，通过响应面法优化其提取工艺.结果表明，乙醇浓度对得率的影响达到极显著水平（P＜0.01），提取时间对得率的影响显著（P＜0.05），而提取温度与料液比对得率的影响不显著；4个因素对得率的影响大小是：乙醇浓度＞提取时间＞提取温度＞料液比.响应面分析得到的回归模型能够较好地预测实际得率，乙醇浸提龙眼核多酚的最优条件为：提取时间106 min，乙醇体积分数为58 %，提取温度64 ℃，料液比为1∶9，在此条件下，多酚得率为42690 mg/g，达到理论预测值的96.29 %

Abstract:: The polyphenols were extracted from the longan by ethanol.On the basis of singlefactor experiments,the optimal extraction conditions were obtained by using the response surface analysis.The results showed that ethanol concentration reached a significant level on the yield(P<0.01),extraction time was significant on yield(P<0.05),while the extraction temperature and solid to liquid ratio was not significant on the yield,the four factors primary and secondary relationship was ethanol concentration,extraction time,extraction temperature,solid to liquid ratio.The practical extraction rate could be well in accordance with the prediction value of the regression model obtained by response surface analysis.And the optimum extraction were as follow:extraction time was 106 min,ethanol concentration was 58 %,extraction temperature was 64 ℃,liquid ratio was 1∶9,and the yield was 42.690 mg/g