鳗鲡养殖循环水处理系统细菌的组成及其数量-《集美大学学报（自然科学版）》

文章信息/Info

Title:: The Composition and the Quantity of Bacteria in a Water Recycling System for Culturing European Eel

作者:: 邓德波1; 2; 马英1; 2; 徐春燕1; 2; 关瑞章1; 2; (1.集美大学水产学院,福建厦门 361021; 2.福建省高校水产科学技术与食品安全重点实验室,福建厦门 361021)

Author(s):: DENG De-bo1; 2; MA Ying1; 2; XU Chun-yan1; 2; GUAN Rui-zhang1; 2; (1.Fisheries College,Jimei University,Xiamen 361021,China;2.Key Laboratory of Science and Technology forAquaculture and Food Safety(Jimei University),Fujian Province,Xiamen 361021,China)

摘要:: ］为研究鳗鲡养殖循环水处理系统中细菌的组成与数量，从循环水处理系统中的养殖池、沉淀池、生物膜池、滤池和紫外消毒池采集水样，并从生物膜池和滤池中采集附着生物样品，分别用平板菌落计数法和16S rRNA基因克隆文库分析法进行细菌计数和附着样品的细菌组成分析.结果表明：1）养殖水经过各处理环节后，水体中细菌数量逐渐减少，如由养殖池中的2.98×106 CFU/L降低至牡蛎壳填料滤池中的9.30×105 CFU/L，再经过紫外消毒池后，水体中细菌数量又减少了96 %，再降低至3.53×104 CFU/L.2）生物膜池的尼龙丝挂网载体和滤池中牡蛎壳填料上附着细菌数量较多，其数量分别高达1.39×108 CFU/g和1.52×108 CFU/g.牡蛎壳上的附着细菌主要分为3大类：分别属于变形菌门（包括α-、β-和γ-变形菌纲）、拟杆菌门和异常球菌-栖热菌门，其中以β-变形菌纲细菌为优势类群，大部分细菌对环境有机物具有良好的分解作用

Abstract:: The aim of this work was to study the composition and quantity of bacterial populations in a water recycling system for culturing European eel.Water samples were collected from each treatment in the experimental aquaculture system,and the attached biological samples were collected from biofilm pool and filter.The composition and the quantity of bacteria were determined by plate count method and 16S rRNA gene clone library analysis.The results showed the number of bacteria decreased gradually with the wastewater treatment procedure，from 2.98×106 CFU/L in breeding pond down to 9.3×105 CFU/L in the oyster filler filtration pond.The number of bacteria decreased by 96 % after UV disinfecting and reduced to 3.5 ×104 CFU/L.The carriers enriched a great number of bacteria.The bacterial quantities in nylon net hanging and oyster shells were up to 1.39×108 CFU/g and 1.52×108 CFU/g,respectively.The attached bacteria on oyster shells could be divided into three major groups: Proteobacteria (including the α-,β-and γ- Proteobacteria),Bacteroidetes,and Deinococcus-Thermus, and the β-Proteobacteria were dominant components,most of which had the ability to degrade environmental organics

[1]郭松林,关瑞章,冯建军,等.基于16S rDNA序列和RFLP分析的病鳗分离菌株鉴定[J].集美大学学报(自然科学版),2012,17(2):81.
[2]白雅婷,吴国宏,许智海,等.PCR-DGGE法分析海参花酶解物对小鼠肠道菌群的影响[J].集美大学学报(自然科学版),2018,23(1):8.
　BAI Yating,WU Guohong,XU Zhihai,et al.The Effects of Feeding Enzymatic Hydrolysate of Sea Cucumber Gonad on Intestinal Microbiota of Mice by PCR-DGGE[J].Journal of Jimei University,2018,23(5):8.