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[1]钟君伟,鄢庆枇,纪荣兴,等.高效好氧反硝化菌的紫外诱变育种[J].集美大学学报(自然科学版),2011,16(1):14-19.
　ZHONG Jun -wei,YAN Qing -pi,JI Rong -xing,et al.Mutation Breeding of Aerobic Denitrifiers by UV -irradiation[J].Journal of Jimei University,2011,16(1):14-19.
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《集美大学学报（自然科学版）》[ISSN:1007-7405/CN:35-1186/N]

卷:
第16卷
期数:
2011年第1期
页码:
14-19
栏目:
水产科学与生物工程
出版日期:
2011-01-25

文章信息/Info

Title:
Mutation Breeding of Aerobic Denitrifiers by UV -irradiation
作者:
钟君伟1; 2; 鄢庆枇1; 2; 纪荣兴1; 2; 朱苏琴1; 2
(1.集美大学水产学院,福建 厦门 361021;2.福建省高校水产科学技术与食品安全重点实验室,福建 厦门 361021)
Author(s):
ZHONG Jun -wei1; 2; YAN Qing -pi1; 2; JI Rong -xing1; 2; ZHU Su -qin1; 2
(1.Fisheries College,Jimei University,Xiamen 361021,China;2.Key Laboratory of Science and Technology forAquaculture and Food Safety(Jimei University),Fujian Province,Xiamen 361021,China)
关键词:
好氧反硝化菌; 紫外诱变; 脱氮
Keywords:
aerobic denitrifier; UV-irradiation; denitrification characteristics
分类号:
-
DOI:
-
文献标志码:
-
摘要:
采用紫外诱变法对好氧反硝化菌A762进行诱变处理，根据显色圈大小(G)与菌落直径(C)之比，初筛得到8株突变菌，再根据脱氮效果，从中复筛出1株总氮（TN）去除率最高的突变株B25，并对其好氧反硝化性能进行了研究.结果显示：紫外诱变96 h后，在好氧条件下，相对于原菌株A762，菌株B25具有更好的生长优势，对NO-3-N去除率达到90 %以上，远高于原菌的22 .201 %；能短时间内去除积累的亚硝态氮，TN去除率提高到84 .627 %，比原菌株A762提高了60 .071 %.在反硝化过程中,培养液pH值逐渐上升,而氧化还原电位(ORP)逐渐降低.虽然诱变株B25有较强的反硝化活性，但还要进一步研究其在养殖水体中的脱氮效果，以便能得到实际应用
Abstract:
An aerobic denitrifier (strain A762) was mutagenized by UV -irradiation and 8 mutants were selected for their higher ratios of G/C.A mutant (B25) with highest rate of TN removal was obtained.The aerobic denitrification characteristics of strains B25 and A762 were investigated.The results showed:compared with strain A762,strain B25 grew fast and had a higher reduction rate of NO-3-N and NO-2-N；during the denitrification process of both strains,the pH values in the culture medium gradually increased,while the Oxidation-reduction Potential gradually decreased.Strain B25 showed its ability in aerobic denitrification,but more studies should be carried out before its application to aquaculture water

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更新日期/Last Update: 2018-06-13