|本期目录/Table of Contents|

[1]潘冰青,刘光明,曹敏杰,等.鲤鱼胶原蛋白的过敏原性研究[J].集美大学学报(自然科学版),2011,16(5):340-345.
　PAN Bing-qing,LIU Guang-ming,CAO Min-jie,et al.IgEStudy on the Allergen of Collagen Isolated fromSkin of Carp(Cyprinus carpio)[J].Journal of Jimei University,2011,16(5):340-345.
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《集美大学学报（自然科学版）》[ISSN:1007-7405/CN:35-1186/N]

卷:
第16卷
期数:
2011年第5期
页码:
340-345
栏目:
水产科学与生物工程
出版日期:
2011-09-25

文章信息/Info

Title:
IgEStudy on the Allergen of Collagen Isolated fromSkin of Carp(Cyprinus carpio)
作者:
潘冰青1; 2; 刘光明1; 2; 曹敏杰1; 2; 蔡秋凤1; 2; 翁武银1; 2; 苏文金1; 2
(1.集美大学生物工程学院,福建 厦门 361021;2.福建省高校水产科学技术与食品安全重点实验室,福建 厦门 361021)
Author(s):
PAN Bing-qing1; 2; LIU Guang-ming1; 2; CAO Min-jie1; 2; CAI Qiu-feng1; 2; WENG Wu-yin1; 2;  SU Wen-jin1; 2
(1.School of Biotechnology Engineering,Jimei University,Xiamen 361021,China;2.Key Laboratory ofScience and Technology for Aquaculture and Food Safety(Jimei University),Fujian Province,Xiamen 361021,China)
关键词:
鲤鱼; 胶原蛋白; 过敏原; 稳定性; IgE结合活性Study on the Allergen of Collagen Isolated fromSkin of Carp(Cyprinus carpio)
Keywords:
Cyprinus carpio; collagen; allergen; stability; specific IgE reactivity
分类号:
-
DOI:
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文献标志码:
-
摘要:
以鲤鱼鱼皮为研究对象，通过碱溶、酸溶、NaCl盐析等方法从鱼皮中分离纯化得到了胶原蛋白.SDS-PAGE分析显示，鲤鱼胶原蛋白由分子质量约为120 ku和115 ku的2条α链（α1与α2）、分子质量约为250 ku的β链以及大分子质量的γ链组成.分析比较鲤鱼胶原蛋白与鲢鱼小清蛋白的基本性质，结果显示：2种蛋白质的pH值稳定性相似；胶原蛋白的热稳定性不如小清蛋白，但比小清蛋白更耐胃蛋白酶消化；在高温高压处理下，胶原蛋白明显降解，小清蛋白则出现了降解与聚合2种情况.应用8份鱼类过敏患者血清的酶联免疫吸附测定实验结果显示，小清蛋白对6份血清有特异性IgE反应，胶原蛋白对 3份血清有特异性IgE反应.Dot-blot实验结果显示，胶原蛋白与鱼类过敏患者血清出现了特异性的杂交显色斑点.与小清蛋白相似，鲤鱼胶原蛋白具有特异的IgE结合活性，提示其是一种潜在的过敏原
Abstract:
Collagen of carp skin was isolated by alkali extraction,acid extraction and NaCl precipitation.Carp collagen consisted of α1 chain(120 ku),α2 chain (115ku),β chain (250 ku),and small amount of γ chain in SDS-PAGE.Analysis of the basic property of collagen and parvalbumin(the major allergen of fish)showed the pH stability of two proteins did not present distinct difference;collagen was less stable to heating treatment,but more resistive to SGF digestion;treated with high temperature and high pressure, collagen was decomposed into fragments,parvalbumin presented decomposition and polymerization simultaneously.ELISA analysis of eight sera from fishallergic patients showed parvalbumin had the specific IgE reactivity to six sera,while collagen had the specific IgE reactivity to three sera.Dot-blot analysis of collagen also presented the specific IgE reactivity to the sera from fish-allergic patients.The results indicated that carp collagen might be a potential allergen

参考文献/References:

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更新日期/Last Update: 2018-06-13