|本期目录/Table of Contents|

[1]李鹤宾,刘光明,陈艳红,等.重组锰超氧化物歧化酶工程菌摇瓶发酵条件的优化[J].集美大学学报(自然科学版),2012,17(1):26-32.
　LI He-bin,LIU Guang-ming,CHEN Yan-hong,et al.Optimization of Fermentation Conditions in a Shaker for the Recombinant Manganese Superoxide Dismutase from Engineering E.coli[J].Journal of Jimei University,2012,17(1):26-32.
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《集美大学学报（自然科学版）》[ISSN:1007-7405/CN:35-1186/N]

卷:
第17卷
期数:
2012年第1期
页码:
26-32
栏目:
水产科学与生物工程
出版日期:
2012-01-25

文章信息/Info

Title:
Optimization of Fermentation Conditions in a Shaker for the Recombinant Manganese Superoxide Dismutase from Engineering E.coli
作者:
李鹤宾1; 刘光明2; 陈艳红2; 黄继福2; 朱艳冰2
(1.厦门医学高等专科学校,福建 厦门 361008;2.集美大学生物工程学院,福建 厦门 361021)
Author(s):
LI He-bin1; LIU Guang-ming2; CHEN Yan-hong2; HUANG Ji-fu2; ZHU Yan-bing2
(1.Xiamen Medical College,Xiamen 361008,China;2.School of Biotechnology Engineering,Jimei University,Xiamen 361021,China)
关键词:
重组锰超氧化物歧化酶; 发酵; 乳糖; 工程菌
Keywords:
recombinant manganese superoxide dismutase; fermentation; lactose;  engineering bacteria
分类号:
-
DOI:
-
文献标志码:
-
摘要:
        通过单因素试验对重组锰超氧化物歧化酶（Recombinant Manganese Superoxide Dismutase，rMn-SOD）工程菌的摇瓶发酵的培养基（碳源、氮源、无机盐）和培养条件（接种量、乳糖浓度、时间、温度、摇床转速、pH值等）进行了初步优化.结果表明，工程菌发酵的优化培养基组分（质量分数）为：1.5 %蛋白胨、1.5 %酵母提取物、1.0 % NaCl、0.4 % KH2PO4、0.8 % K2HPO4、1.5 %（体积分数）甘油、0.2 % NH4Cl和5 mmol/L MnCl2.乳糖诱导表达的优化条件为：以5 %接种量培养5 h后，加入质量分数为0.25 %的乳糖进行诱导表达6 h.当发酵温度为37 ℃、摇床转速为180 r/min、培养基的初始pH值为7.0时，表达的rMn-SOD的酶活力高.在优化条件下，工程菌的SOD活性达1 969.9 U/mL，比活力达1 081.8 U/mg
Abstract:
Fermentation media(carbon source,nitrogen source,inorganic salt)and cultivation conditions(inoculum quantity,lactose concentration,time,temperature,shaker speed,pH value etc.) by single factor researches were preliminarily optimized in a shaker for the recombinant manganese superoxide dismutase from engineering E.coli.The results showed that the optimized culture media for the engineering E.coli were 1.5 % trytone,1.5 % yeast extract,1.0 % NaCl,0.4 % KH2PO4,0.8 % K2HPO4,1.5 % glycerine,0.2 % NH4Cl and 5 mmol/L MnCl2.The optimized lactose induction conditions were growth for 5 h after inoculation at 5 % inoculum quantity,and then adding 0.25 % lactose to induce for 6 h.The enzyme specific activity was the highest when the fermentation temperature was 37 ℃,the shaker speed was 180 r/min,and the original medium pH value was 7.0.Under all of these optimized conditions,SOD from the engineering E.coli exhibited the enzymatic activity of 1 969.9 U/mL and the specific activity of 1 081.8 U/mg

参考文献/References:

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备注/Memo

备注/Memo:
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更新日期/Last Update: 2014-06-28