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[1]杨秋明,许海琴,倪辉,等.利用高效液相色谱检测发酵液中S-腺苷蛋氨酸含量[J].集美大学学报(自然科学版),2012,17(6):421-427.
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利用高效液相色谱检测发酵液中S-腺苷蛋氨酸含量(PDF)
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《集美大学学报（自然科学版）》[ISSN:1007-7405/CN:35-1186/N]

卷:
第17卷
期数:
2012年第6期
页码:
421-427
栏目:
水产科学与生物工程
出版日期:
2012-11-25

文章信息/Info

Title:
-
作者:
杨秋明1; 2; 3; 许海琴1; 倪辉1; 2; 3; 蔡慧农1; 2; 3; 肖安风1; 2; 3
(1.集美大学生物工程学院,福建 厦门 361021;2.福建省高校食品微生物与酶工程研究中心,福建 厦门 361021;3．厦门市食品生物工程技术研究中心,福建 厦门 361021)
Author(s):
-
关键词:
高效液相色谱; S-腺苷蛋氨酸; 检测
Keywords:
HPLC; S-adenosylmethionine; determination
分类号:
-
DOI:
-
文献标志码:
-
摘要:
        建立了利用Waters2695高效液相色谱测定毕赤酵母发酵液中S-腺苷蛋氨酸（S-adenosylmethionine,SAM)含量的方法，对HPLC-UV法检测SAM含量的色谱条件进行优化，最佳色谱条件如下：Alltima C18柱，150 mm×4.6 mm,5 μm,流动相为V(0.01 mmol/L甲酸铵）∶V（甲醇）=97∶3，流速0.6 mL/min，柱温30 ℃，检测波长260 nm.优化后的色谱分离条件可使样品中的SAM与杂质达到较佳分离，稳定性、精密度及重复性都较好，其RSD均小于1.5%.利用SAM标品制作标准曲线Y=32408606.0721 X+130181.2824，线性范围为0.012~1.2 g/L，R2达到0.9998，平均回收率为100.027%~102.533%.此方法可有效地检测发醇液中S-腺苷蛋氨酸含量
Abstract:
An HPLC determination method was developed for measuring S-adenosylmethionine (SAM) contents in culture broth of Pichia pastoris.The optimized HPLC conditions for SAM analysis were 0.01 mmol/L ammonium formate solution (pH=3.5)-methylalcohol(V∶V=97∶3) as mobile phase,flow rate 0.6 mL/min,column temperature 30 ℃,and detection wavelength 260 nm.The separation conditons described above achieved good separation effect,together with high stability,sensitivity,and reproducibility.The standard curve of SAM was Y=32408606.0721 X+130181.2824，and the linear range was 0.012~1.2 g/L,R2 was 0.9998,the average recycle rate was 100.027%~102.533%.This method would be useful for SAM analysis during Pichia pastoris fermentation

参考文献/References:

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更新日期/Last Update: 2014-06-28