花鳗鲡病原菌弗氏柠檬酸杆菌的鉴定-《集美大学学报（自然科学版）》

文章信息/Info

Title:: Identification of Pathogenic Citrobacter freundii Isolated from Anguilla marmorata

作者:: 杨方园¹; 关瑞章¹; 2; 3; 李忠琴¹; 2; 3; 于海振¹; 李秋云¹; (1.集美大学水产学院,福建厦门 361021 ;2.农业部东海海水健康养殖重点实验室,福建厦门 361021 ;3.集美大学鳗鲡现代产业技术教育部工程研究中心,福建厦门 361021 )

Author(s):: YANG Fang-yuan¹; GUAN Rui-zhang¹; 2; 3; LI Zhong-qin¹; 2; 3; YU Hai-zhen¹; LI Qiu-yun¹; (1.Fisheries Colleges，Jimei University，Xiamen 361021，China；2.Key Laboratory of Healthy Aquaculture of East China Sea，Ministry of Agriculture，Xiamen 361021，China；3.Engineering Research Center of theModern Industrial Technology for Eel，Ministry of Education，Jimei University,Xiamen 361021，China)

Keywords:: Anguilla marmorata; Citrobacter freundii; antibiotic sensitivity; pathogenic bacterium

摘要:: 首次报道了从养殖花鳗鲡（Anguilla marmorata）肝脏中分离得到优势菌株AMRB6，经人工腹腔注射感染健康花鳗鲡试验，结果证实该菌有致病作用，其半数致死量LD₅₀为3.2×107 CFU/ mL.结合菌体形态特征、常规生理生化鉴定和Biolog自动微生物鉴定系统鉴定，以及16S rRNA序列扩增结果，表明该菌为弗氏柠檬酸杆菌（Citrobacter freundii）.30种药物药敏试验结果表明，该菌株对左氧氟沙星、诺氟沙星、庆大霉素、卡那霉素等14种药物高度敏感

Abstract:: The paper was the first one reported that a pathogenic bacterium，named AMRB6，was isolated from the liver of diseased Anguilla marmorata.The strain was proven to be a pathogen of eel by intraperitoneal injection with bacteria suspension ，and the LD50 was 3.2×107CFU/mL.Based on the morphological characters of bacteria，traditional physiological and biochemical identification and Biolog identification system ，and analysis of 16S rRNA gene ，the strain was identified to be Citrobacter freundii．In antibiotic sensitivity test to 30 antimicrobial agents，the stain had high sensitivity to 14 agents including levofloxacin，norfloxacin，gentamicin，and kanamycin