|本期目录/Table of Contents|

[1]欧柳舒,沈建东,翁凌,等.鲍鱼性腺小肽的制备及抗氧化活性的初步研究[J].集美大学学报(自然科学版),2014,19(1):13-19.
　OU Liu-shu,SHEN Jian-dong,WENG Ling,et al.Antioxidant Activity of Enzymatic Hydrolysates Prepared from Pacific Abalone (Haliotis discus hannai) Gonads[J].Journal of Jimei University,2014,19(1):13-19.
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鲍鱼性腺小肽的制备及抗氧化活性的初步研究()
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《集美大学学报（自然科学版）》[ISSN:1007-7405/CN:35-1186/N]

卷:
第19卷
期数:
2014年第1期
页码:
13-19
栏目:
水产、食品与生物工程
出版日期:
2014-01-25

文章信息/Info

Title:
Antioxidant Activity of Enzymatic Hydrolysates Prepared from Pacific Abalone (Haliotis discus hannai) Gonads
作者:
欧柳舒1; 2; 沈建东1; 2; 翁凌1; 2; 陶志鹏1; 2; 张凌晶1; 2; 曹敏杰1; 2
1.集美大学生物工程学院，福建 厦门 361021;2.福建省水产品深加工工程研究中心，福建 厦门 361021
Author(s):
OU Liu-shu1; 2; SHEN Jian-dong1; 2; WENG Ling1; 2; TAO Zhi-peng1; 2; ZHANG Ling-jing1; 2; CAO Min-jie1; 2
1.College of Biological Engineering,Xiamen 361021,China;2.Engineering Research Center for
Aquatic Products Processing of Fujian Province,Jimei University,Xiamen 361021,China
关键词:
鲍鱼; 性腺; 酶解条件; 抗氧化活性肽; 蛋白酶
Keywords:
abalone; gonads; hydrolyzing conditions; antioxidant active peptides; proteinase
分类号:
-
DOI:
-
文献标志码:
A
摘要:
以雄性皱纹盘鲍性腺为原料，建立了蛋白酶酶解制备抗氧化活性蛋白肽的工艺条件.分别对木瓜蛋白酶和中性蛋白酶进行单因素研究，并以二者的复合酶为工具酶进行响应面分析.结果显示，雄性鲍鱼性腺的最优酶解条件为木瓜蛋白酶与中性蛋白酶的比例为1∶4、pH=6.6、酶解温度为53.7 ℃、酶解时间为70.4 min.高效液相色谱（HPLC）分析结果表明，酶解液中多肽的分子质量主要分布在1 ku以下.制备的酶解液具有较强的清除自由基效果，清除1,1-二苯基-2-三硝基苯肼（DPPH）的EC50值为6.8 mg/mL，还原力的AC0.5值为12.87 mg/mL.
Abstract:
Using male abalone gonads as raw material,the present study optimized the proteinase hydrolyzing conditions to produce antioxidant peptides.Single-factor experiments using papain and neutral proteinase were adopted respectively and the combination of both enzymes was performed.The results showed that the optimal hydrolyzing conditions for preparation of active antioxidant peptides from male abalone gonads were using the proportion of papain to neutral proteinase at 1∶4,at pH=6.6 and 53.7 ℃,with hydrolyzing duration of 70.4 min.High performance liquid chromatography (HPLC) analysis showed that the molecular mass of peptides in the hydrolysate was mostly below 1 ku.The hydrolysate thus obtained from male abalone gonads revealed a significant effect in scavenging free radicals.The half scavenging effect (EC50) of DPPH radical was 6.8 mg/mL and the half absorbance effect (AC0.5) of reducing power was 12.87 mg/mL.

参考文献/References:

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备注/Memo:
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更新日期/Last Update: 2014-12-01