促进剂对枯草芽胞杆菌CICC20958产肌苷的影响-《集美大学学报（自然科学版）》

文章信息/Info

Title:: Effect of Accelerators on Inosine Production of Bacillus subtilis CICC20958

作者:: 游洪燕¹; 2; 杨哲¹; 肖安风¹; 2; 3; 4; 倪辉¹; 2; 3; 4; 蔡慧农¹; 2; 3; 4; 杨秋明¹; 2; 3; 4; 1.集美大学生物工程学院,福建厦门361021;2.福建省食品微生物与酶工程重点实验室,福建厦门 361021;3.福建省高校食品微生物与酶工程技术研究中心,福建厦门361021;4.厦门市食品生物工程技术研究中心,福建厦门361021

Author(s):: YOU Hong-yan¹; 2; YANG Zhe¹; XIAO An-feng¹; 2; 3; 4; NI Hui¹; 2; 3; 4; CAI Hui-nong¹; 2; 3; 4; YANG Qiu-ming¹; 2; 3; 4; 1.College of Biological Engineering,Jimei University,Xiamen 361021,China;2.Fujian Provincial Key Laboratory of Food Microbiology and Enzyme Engineering,Xiamen 361021,China;3.Research Center of Food Microbiology and Enzyme Engineering Technology of Fujian Province,Xiamen 361021,China;4.Research Center of Food Biotechnology of Xiamen City,Xiamen 361021,China

Keywords:: Bacillus subtilis; CICC20958; inosine; accelerator; uniform design

摘要:: 根据肌苷的代谢调控机理，选择柠檬酸钠、NaF、CaCl2、次黄嘌呤、MnSO4 5种肌苷促进剂，考察它们的不同添加量对枯草芽胞杆菌（Bacillus subtilis,简称Bs）CICC20958菌株产肌苷的影响，采用均匀设计法得到最佳组合添加方案.结果表明，在促进剂柠檬酸钠、NaF、CaCl₂、次黄嘌呤、MnSO4质量浓度分别为7.9、0.001、0.032、2.1、0.2 g/L的条件下发酵，菌株Bs CICC20958的肌苷产量达到7.81 g/L，比不添加促进剂实验组（对照组）的肌苷产量提高了5.38倍.

Abstract:: According to the regulating mechanism of inosine synthesis,some kinds of effective additives(sodium citrate,NaF,CaCl₂,hypoxanthine and MnSO₄ ) were added in Bacillus subtilis CICC20958.Then,the best composition of accelerators was obtained by uniform design.The results showed that optimized combination increased inosine production to 7.81 g/L in the presence of 7.9 g/L sodium citrate,0.001 g/L NaF,0.032 g/L CaCl₂,2.1 g/L hypoxanthine and 0.2 g/L MnSO₄.The yield of inosine was 5.38 times higher than that of the control group.