|本期目录/Table of Contents|

[1]马芮萍,朱艳冰,倪辉,等.海洋细菌NTa发酵产琼胶酶条件的初步优化[J].集美大学学报(自然科学版),2014,19(4):259-265.
　MA Rui-ping,ZHU Yan-bing,NI Hui,et al.Optimal Cultivation of a Marine Bacterium NTa for Agarase Production[J].Journal of Jimei University,2014,19(4):259-265.
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海洋细菌NTa发酵产琼胶酶条件的初步优化()
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《集美大学学报（自然科学版）》[ISSN:1007-7405/CN:35-1186/N]

卷:
第19卷
期数:
2014年第4期
页码:
259-265
栏目:
水产、食品与生物工程
出版日期:
2014-07-25

文章信息/Info

Title:
Optimal Cultivation of a Marine Bacterium NTa for Agarase Production
作者:
马芮萍; 朱艳冰; 倪辉; 肖安风; 蔡慧农
?集美大学生物工程学院，福建 厦门 361021
Author(s):
MA Rui-ping; ZHU Yan-bing; NI Hui; XIAO An-feng; CAI Hui-nong
College of Biological Engineering，Jimei University，Xiamen 361021,China
关键词:
琼胶酶; Stenotrophomonas sp.; 发酵条件
Keywords:
agarase; Stenotrophomonas sp.; fermentation condition
分类号:
-
DOI:
-
文献标志码:
A
摘要:
利用选择性培养基从厦门红树林泥土样品中分离得到一株具有较高琼胶酶活力的海洋细菌NTa，通过16S rRNA分析，将该菌株归属为Stenotrophomonas sp.NTa.对该菌株发酵产琼胶酶的条件进行初步优化，结果表明：菌株NTa产琼胶酶的最佳碳源是琼脂，氮源是酵母浸膏，NaCl的质量分数为5%，在28 ℃发酵培养40 h，发酵液的酶活力达到1.98 U/mL，比优化前提高了37.35%.
Abstract:
An agarase-producing bacterium NTa was isolated from coastal sediment in Xiamen by selective medium.The agar-degrading bacterium NTa was classified as the genus of Stenotrophomonas sp.based on 16S rRNA analysis.With one-factor at a time method,the optimal fermentation conditions were confirmed.The results showed that for optimal growth of the agarase-producing bacterium NTa,agar and yeast were used as carbon and nitrogen sources,at 5% of NaCl concentration and 28 ℃.After cultivation under the above conditions for 40 h,the production of agarase reached the peak with an activity of 1.98 U/mL,which was 37.35% higher than that performed previously.

参考文献/References:

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相似文献/References:

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　GAO He,WANG Xin-xia,NI Hui,et al.Cloning and Bioinformatics Analysis of the Agarase Gene from Microbulbifer sp.AG1[J].Journal of Jimei University,2016,21(4):261.
[2]邵嫄,姚德恒,洪清林,等.海洋弧菌NTi产琼胶酶的发酵条件优化[J].集美大学学报(自然科学版),2019,24(1):20.
　SHAO Yuan,YAO Deheng,HONG Qinglin,et al.Process Optimization of Agarases Fermentation by Vibrio natriegens NTi[J].Journal of Jimei University,2019,24(4):20.
[3]陈艳红,王海琪,马芮萍,等.响应面法优化海洋细菌NTa产琼胶酶的发酵条件[J].集美大学学报(自然科学版),2021,26(3):206.
　CHEN Yanhong,WANG Haiqi,MA Ruiping,et al.Response Surface Optimization of Fermentation Conditions for Agarase Production by Marine Bacterium NTa[J].Journal of Jimei University,2021,26(4):206.

备注/Memo

备注/Memo:
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更新日期/Last Update: 2014-12-02