一株耐镍寡养单胞菌的分离及鉴定-《集美大学学报（自然科学版）》

文章信息/Info

Title:: Isolation and Characterization of a Nickel-resistant Stenotrophomonas Strain

作者:: 王鹤1; 2; 巫晶晶1; 2; 张金丽1; 2; 王洁1; 2; 贾成光1; 2; 张亚平1; 2; （1.集美大学食品与生物工程学院，福建厦门 361021； 2.福建省食品微生物与酶工程重点实验室，福建厦门 361021）

Author(s):: WANG He1; 2; WU Jing-jing1; 2; ZHANG Jin-li1; 2; WANG Jie1; 2; JIA Cheng-guang1; 2; ZHANG Ya-ping1; 2; （1.College of Food and Biological Engineering,Jimei University,Xiamen 361021,China；2.Fujian Provincial Key Laboratory of Food Microbiology and Enzyme Engineering,Xiamen 361021,China）

摘要:: 从福建某五金厂综合污水中筛选到一株对Ni2+耐受性400 mg/L的菌株，对其形态学、16S rDNA序列及生理生化特征进行分析，并利用较佳生长条件对所得菌株进行Ni2+吸附验证.结果表明：该菌为革兰氏阴性杆菌，在牛肉膏蛋白胨培养基上形成的菌落，表面较光滑，边缘整齐；经16S rDNA序列比对及生理生化特征的分析，将菌株 JMUZJ-1鉴定为寡养单胞菌（Stenotrophomonas sp.）；菌株JMUZJ-1生长的较好条件是pH 7.0，温度30 ℃，NaCl质量分数0.5%，在该条件下，菌株对Ni2+表现出良好的吸附性能.

Abstract:: A nickel resistant strain was obtained from a hardware factory in Fujian province by selection of spontaneous mutants in wastewater containing Ni2+ .Morphological tests,molecular identifications of 16S rDNA and biochemical characterization to this strain have been performed.The efficiency of the strain in absorbing Ni2+ under optimal growing conditions has been analyzed.The results showed that the nickel?-resistant strain was Gram-negative.The strain produces yellow pigmented colonies on medium including beef extract and peptone,with smooth surface and neat edge.Sequence analysis of 16S rDNA and biochemical characteristics identified the strain JMUI-1 as Stenotrophomonas sp.The optimal growth condition for the nickel resistant strain is at pH 7.0,30 ℃,containing 0.5% NaCl solution,which allows JMUI-1 a good absorption performance of Ni2+.