|本期目录/Table of Contents|

[1]杨秋明,李靖雅,肖安风,等.柚皮中柚皮苷和类柠檬苦素的提取及分离纯化[J].集美大学学报(自然科学版),2015,20(6):414-420.
　YANG Qiu-ming,LI Jing-ya,XIAO An-feng,et al.Extraction and Purification of Naringin and Limonoids from Pomelo Peel[J].Journal of Jimei University,2015,20(6):414-420.
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《集美大学学报（自然科学版）》[ISSN:1007-7405/CN:35-1186/N]

卷:
第20卷
期数:
2015年第6期
页码:
414-420
栏目:
水产、食品与生物工程
出版日期:
2015-11-25

文章信息/Info

Title:
Extraction and Purification of Naringin and Limonoids from Pomelo Peel
作者:
杨秋明1; 2; 3; 4; 李靖雅1; 肖安风1; 2; 3; 4; 陈俊1; 2; 3; 4
(1.集美大学食品与生物工程学院，福建 厦门 361021；2.福建省食品微生物与酶工程重点实验室，福建 厦门 361021；3.厦门市食品生物工程技术研究中心，福建 厦门 361021；4.厦门南方海洋研究中心经济海藻资源化利用与深加工重点实验室，福建 厦门 361021)
Author(s):
YANG Qiu-ming1; 2; 3; 4; LI Jing-ya1; XIAO An-feng1; 2; 3; 4; CHEN Jun1; 2; 3; 4
(1.College of Food and Biological Engineering,Jimei University,Xiamen 361021,China；2.Key Laboratory of Food Microbiology and Enzyme Engineering of Fujian Province,Xiamen 361021,China；3.Research Center of Food Biotechnology of Xiamen,Xiamen 361021,China；4.Key Laboratory of Recycling Application and Deep Processing in Economic MarineAlga,Xiamen South Oceanographic Research Center,Xiamen 361021,China)
关键词:
柚皮苷; 柠檬苦素类似物; 大孔吸附树脂; 分离纯化
Keywords:
naringin; limonoids; macro porous resin; separation and purification
分类号:
-
DOI:
-
文献标志码:
A
摘要:
用热水煮提法提取出柚皮中柚皮苷和类柠檬苦素等大量苦味活性物质，通过静态吸附－解吸试验，筛选出具有较大吸附和解吸率的D101大孔吸附树脂进行分离纯化，得出最优的吸附条件：树脂添加量3.0%（质量分数），25 ℃恒温条件下摇床以180 r/min振荡90 min.在此条件下，柚皮苷和柠檬苦素吸附率都达到100%.吸附完全后进行动态洗脱，洗脱液再通过萃取和重结晶后可有效分离纯化柚皮苷和柠檬苦素类似物，纯化后的样品用HPLC法测得柚皮苷纯度达到95.8%，用紫外分光光度法测得柠檬苦素类似物纯度达到90.9%.
Abstract:
Bitterness active components such as naringin and limonoids were extracted by heating water.By means of adsorption-desorption experiments,the macro porous resin D101 was selected for isolation and purification of naringin and limonoids from pomelo peel due to its higher adsorption and desorption rate.The adsorption condition was optimized in the presence of 3% resin with absorption time of 90 min,at 25 ℃ and rotation speed of 180 r/min.After complete adsorption,the resulting solution was applied to dynamic-state elution.Naringin and limonoids were separated and purified effectively via concentration of the eluent and recrystalization.The samples thus prepared were determined by HPLC and UV spectrophotometer,the purities of naringin and limonoids reached 95.8% and 90.9%,respectively.

参考文献/References:

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备注/Memo

备注/Memo:
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更新日期/Last Update: 2015-12-16