琼脂硫酸酯酶酶制剂的制备工艺及其贮藏性能-《集美大学学报（自然科学版）》

文章信息/Info

Title:: Production of Solid Agar Sulfatase and Storage Stability of Enzyme Preparation

作者:: 田友明1; 孙元亨1; 肖琼1; 2; 3; 4; 朱艳冰1; 2; 3; 4; 吴昌正1; 2; 3; 4; 肖安风1; 2; 3; 4; (1.集美大学食品与生物工程学院，福建厦门 361021；2.福建省食品微生物与酶工程重点实验室，福建厦门 361021；3.福建省海洋功能食品工程技术研究中心，福建厦门 361021；4.厦门市海洋功能食品重点实验室，福建厦门 361021)

Author(s):: TIAN Youming1; SUN Yuanheng1; XIAO Qiong1; 2; 3; 4; ZHU Yanbing1; 2; 3; 4; WU Changzheng1; 2; 3; 4; XIAO Anfeng1; 2; 3; 4; (1.College of Food and Biological Engineering,Jimei University,Xiamen 361021,China；2.Key Laboratory of Food Microbiology and Enzyme Engineering of Fujian Province,Xiamen 361021,China；3.Fujian Provincial Engineering Technology Research Center of Marine Functional Food,Xiamen 361021,China；4.Xiamen Key Laboratory of Marine Functional Food,Xiamen 361021,China)

Keywords:: agar sulfatase; spray-drying; enzyme preparation; enzyme activity; storage performance

摘要:: 以重组大肠杆菌（Escherichia.coli）发酵获得的能特异性水解琼脂硫酸酯的重组芳香基硫酸酯酶为研究对象，以酶活力为指标，利用单因素试验对喷雾干燥法制备琼脂硫酸酯酶酶制剂的工艺参数进行优化，并对琼脂硫酸酯酶贮藏过程中各影响因素进行了研究。结果表明，最佳的喷雾干燥工艺条件为：利用麦芽糊精作为助干剂，海藻糖为保护剂，麦芽糊精质量分数25%，进风温度130 ℃，进料速度400 mL/h，热风流量3.5 m3/min，喷雾压力0.25 MPa。然后对制备得到的琼脂硫酸酯酶酶制剂的贮藏稳定性进行考察，发现温度、空气对琼脂硫酸酯酶固体酶制剂的贮藏稳定性影响较大。分别在4 ℃和28 ℃贮藏91 d，4 ℃贮藏的固体酶制剂的酶活力保留率为71.6%，而在28 ℃贮藏的固体酶制剂酶活力保留率为63%。真空条件下28 ℃贮藏91 d，酶活力保留率为65.2%，而敞口条件下为38.3%。光照对琼脂硫酸酯酶酶制剂贮藏也有一定的影响，4 ℃下避光保存91 d，酶活力保留率为72.3%；而不避光贮藏的酶活力保留率只有48.2%。说明，琼脂硫酸酯酶固体酶制剂适宜在4 ℃、真空、避光条件下贮藏。

Abstract:: Arylsulfatase specifically hydrolyzing agar sulfate was obtained from recombinant Escherichia coli.Then,the spray-drying process for enzyme preparation was optimized by the single factor experiments.The optimum spray-drying conditions were determined as follows:25% maltodextrin as drying aid,inlet air temperature 130 ℃,hot air flow rate 3.5 m3/min,feeding rate 400 mL/h,and spray pressure 0.25 MPa,respectively.Then,the appropriated storage method of enzyme preparation was investigated.It showed that the facts of temperature,and air had great influence on the storage stability of enzyme preparation.The solid enzyme was stored at 4 ℃ and 28 ℃ for 91 d separately,and the residual enzyme activities were 71.6% and 63%,respectively.The activity of solid enzyme remained 65.2% kept at 28 ℃ with sealed for 91 d,while the rediual activity of exposed enzyme was 38.3%.In addition,the storage stability of enzyme preparation also was affected by light.The activity of solid enzyme remained 72.3% after stored at 4 ℃ in dark place for 91 d,whereas the rediual activity of exposed enzyme was only 48.2%.Based on the experimental results,the solid enzyme was suited to be stored at 4 ℃,vacuum and dark place.