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[1]田良,陈政强.饲料中添加微生物制剂对仿刺参生长与免疫的影响[J].集美大学学报(自然科学版),2017,22(2):1-10.
　TIAN Liang,CHEN Zhengqiang.Effects of Dietary Probiotics on the Growth and Innate Immunity of Sea Cucumber Apostichopus japonicus[J].Journal of Jimei University,2017,22(2):1-10.
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《集美大学学报（自然科学版）》[ISSN:1007-7405/CN:35-1186/N]

卷:
第22卷
期数:
2017年第2期
页码:
1-10
栏目:
水产、食品与生物工程
出版日期:
2017-03-28

文章信息/Info

Title:
Effects of Dietary Probiotics on the Growth and Innate Immunity of Sea Cucumber Apostichopus japonicus
作者:
田良1; 2; 陈政强1; 2
(1.集美大学水产学院，福建 厦门 361021；2.福建省高校水产科学技术与食品安全重点实验室，福建 厦门 361021）
Author(s):
TIAN Liang1; 2; CHEN Zhengqiang1; 2
(1.Fisherise College,Jimei University,Xiamen 361021,China;2.Key Laboratory of Science and Technology for Aquaculture and Food Safety(Jimei University）,Fujian Province,Xiamen 361021,China)
关键词:
仿刺参; 微生物制剂; 特定生长率; 先天性免疫因子
Keywords:
Apostichopus japonicus; probiotics; specific growth rate; innate immunity factor
分类号:
-
DOI:
-
文献标志码:
A
摘要:
从仿刺参消化道微生物中分离、培育产酶益生菌，解淀粉芽孢杆菌X3-3、甲基营养型芽孢杆菌X4-1和巨大芽孢杆菌X4-5，并制成1.0×109 cfu/mL菌悬液，按1%（质量比）剂量浸泡饲料进行仿刺参饲养实验，并检测分析仿刺参的特定生长率和消化酶活性以及免疫相关因子，以探讨微生物制剂的使用效果。实验结果表明：解淀粉芽孢杆菌X3-3无论是单独使用还是与巨大芽孢杆菌X4-5或/和甲基营养型芽孢杆菌X4-1联合使用皆能显著(P＜0.05)提升仿刺参的SGR和消化酶活性，但饲养30 d和60 d的检测结果差异不明显(P＞0.05)；3株菌制剂的联合使用在显著提升仿刺参体腔液AKP、 ACP、 SOD、 LSZ和POD等体液免疫相关因子活性水平方面的效果优于任意两株菌的搭配使用(P＜0.05)，而任意 2株菌搭配使用的效果则优于除解淀粉芽孢杆菌X3-3之外的单一菌制剂(P＜0.05)。综合分析表明，3株菌联合使用的复合菌制剂使用效果最好，它能显著提升仿刺参特定生长率、消化酶活性和免疫相关因子水平，具有较好的开发应用价值。
Abstract:
In this paper,the effects of dietary probiotics Bacillus amyloliquefaciens X3-3,Bacillus methylotrophicus X4-1 and Bacillus megaterium X4-5 on the growth and innate immunities of sea cucumber Apostichopus japonicus were investigated.The probiotic bacteria were suspended to a density of 1.0×109 cfu/mL and the microbial preparation was added in the diets with a dose of 1%(m/m) for a cultural period of 60 days. The specific growth rates(SGR) and activities of digestive enzymes in digestive tract and non-specific immune effectors in coelomic fluid of the echinoderm subjects were detected and analysed.Results showed that digestive enzymes activities and the specific growth rate (SGR) values of the sea cucumber increased obviously(P＜0.05) no matter Bacillus amyloliquefaciens X3-3 was added alone or combined with strains Bacillus megaterium X4-5 and/or Bacillus methylotrophicus X4-1.However,the results were not significantly different (P>0.05) on 30 d and 60 d. Compared with the combination of any two trains,the combination of three above strains used in the diets had significantly (P<0.05) improved the levels of AKP,ACP,SOD,LSZ and POD involved in sea cucumber humoral immune effectors,and the combination of any two strains was better than the single preparation of either Bacillus methylotrophicus X4-1 or Bacillus megaterium X4-5.Comprehensive analysis indicated that the combination of above three strains used as feed supplement for sea cucumber could significantly increase the values of SGR,digestive enzymes activities and immune related factors of sea cucumbers,and would have potential application values.

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更新日期/Last Update: 2017-05-19