泡叶藻聚糖脱色工艺的研究-《集美大学学报（自然科学版）》

文章信息/Info

Title:: Study on Decolorization of Sulfated Polysaccharide Isolated from Brown Alga Ascophyllum nodosum

作者:: 韦敬柳乙1; 陈艳红1; 2; 3; 4; 杜希萍1; 2; 3; 4; 倪辉1; 2; 3; 4; 朱艳冰1; 2; 3; 4; 肖安风1; 2; 3; 4; 蔡慧农1; 2; 3; 4; 姜泽东1; 2; 3; 4; （1.集美大学食品与生物工程学院，福建厦门 361021；2.厦门南方海洋研究中心经济海藻资源化利用重点实验室，福建厦门361021；3.福建省食品微生物与酶工程重点实验室，福建厦门361021；4.厦门市食品生物工程技术研究中心，福建厦门 361021）

Author(s):: WEI Jingliuyi1; CHEN Yanhong1; 2; 3; 4; DU Xiping1; 2; 3; 4; NI Hui1; 2; 3; 4; ZHU Yanbing1; 2; 3; 4; XIAO Anfeng1; 2; 3; 4; CAI Huinong1; 2; 3; 4; JIANG Zedong1; 2; 3; 4; （1.College of Food and Biological Engineering,Jimei University,Xiamen 361021,China;2.Key Laboratory of Systemic Utilization and Indepth Processing of Economic Seaweed,Xiamen Southern Ocean Technology Center of China,Xiamen 361021,China;3.Fujian Provincial Key Laboratory of Food Microbiology and Enzyme Engineering Technology,Xiamen 361021,China;4.Research Center of Food Biotechnology of Xiamen City,Xiamen 361021,China）

Keywords:: ascophyllan; decolorization optimization; whiteness value; retention rate; activity

摘要:: 为了选取最优的脱色工艺以脱除泡叶藻（Ascophyllum nodosum）聚糖发色基团或物质，通过比较活性炭吸附、有机溶剂（甲醇、乙醇、异丙醇、丙酮和四氢呋喃）萃取、大孔树脂（D101、D101-1、DM130）吸附、H2O2氧化等不同脱色方法对泡叶藻聚糖脱色效果的影响，研究结果表明，H2O2氧化法优于大孔树脂吸附法、活性炭吸附法和有机溶剂萃取法。进一步以脱色时间、脱色温度、H2O2质量分数和脱色pH值为因素，进行单因素试验确定关键影响因素及其水平，并采用L9(34)正交设计进行试验，以泡叶藻聚糖的白度值和脱色后聚糖保留率为指标，并分析脱色后的泡叶藻聚糖对诱导小鼠吞噬细胞RAW264.7生成一氧化氮活性的影响，筛选出具有较好保留泡叶藻聚糖免疫诱导潜力的H2O2氧化法的脱色工艺条件。结果表明，H2O2氧化法脱除泡叶藻聚糖发色基团或物质最佳工艺条件为：H2O2质量分数8.6%，脱色时间4.0 h，脱色温度60 ℃，pH值11.0，泡叶藻聚糖白度值达到（61.05±1.12）%，提高了4.1倍，聚糖保留率为（90.02±0.03）%。

Abstract:: In order to obtain the optimum decolorization method for removing the chromophoric group or substances in ascophyllan,the efficiencies of various decolorization methods with different materials including activated carbon,organic solvent(methanol,ethanol,isopropanol,acetone,and tetrahydrofuran),macroporous resin(D101,DM130,and D101-1),and H2O2 were compared.The results showed that the H2O2decolorization method was more efficient than other methods.To further determine the optimum decolorization technologic parameters of H2O2decolorization method,the quantitative effects of times,temperatures,H2O2 concentrations,and pH values on the whiteness value and the retention rate of decolored ascophyllan were investigated using a single factor experiment method and a L9(34）orthogonal design experiment method,then the activities of decolored ascophyllans on NO production in RAW264.7 cells were analyzed.The results showed that the optimum decolorization technologic parameters were as follows:the H2O2 concentration 8.6%,the decoloring time 4.0 h,the decoloring temperature 60 ℃,and the pH 11.0.Under this condition,the whiteness value of ascophyllan was（61.05±1.12）% increased by 4.1 times,and its retention rate reached to（90.02±0.03）%.