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《集美大学学报（自然科学版）》[ISSN:1007-7405/CN:35-1186/N]

卷:

第23卷

期数:

2018年第1期

页码:

17-26

栏目:

水产、食品与生物工程

出版日期:

2018-01-28

文章信息/Info

Title:

Development of Agar Extraction with Enzymereplacingalkali Process

作者:

翁惠芬1; 2; 3; 肖琼1; 2; 3; 洪清林1; 3; 郭东旭1; 3; 倪辉2; 4; 姜泽东2; 4; 杨秋明2; 4; 肖安风2; 4

（1.福建省绿麒食品胶体有限公司，福建 漳州 363100；2.集美大学食品与生物工程学院，福建 厦门 361021；3.福建省海藻多糖企业工程技术研究中心，福建 漳州 363100；4.福建省海洋功能食品工程技术研究中心，福建 厦门 361021）

Author(s):

WENG Huifen1; 2; 3; XIAO Qiong1; 2; 3; HONG Qinglin1; 3; GUO Dongxu1; 3; NI Hui2; 4; JIANG Zedong2; 4; YANG Qiuming2; 4; XIAO Anfeng2; 4

（1.Fujian Province Lvqi Food Colloid Co.,Ltd,Zhangzhou 363100,China；2.College of Food and Biological Engineering,Jimei University,Xiamen 361021,China;3.Fujian Algal Polysaccharides Engineering Technology Research Center,Zhangzhou 363100,China;4.Fujian Provincial Engineering Technology Research Center of Marine Functional Food,Xiamen 361021,China）

关键词:

琼脂; 酶法提取; 复合酶法提取; 中试放大

Keywords:

agar; enzyme extraction; composite enzyme; scale up

分类号:

-

DOI:

-

文献标志码:

A

摘要:

为了减少琼脂提取过程中的碱用量，开发琼脂的绿色清洁生产工艺，以凝胶强度为考察指标，通过单因素试验对酶法替代碱法提取琼脂的工艺进行了优化。结果表明，酶法辅助琼脂提取工艺最优条件为：碱质量分数3%，处理时间2.5 h，处理温度45 ℃，pH值 7.0，加酶量4 U/mL；复合酶法工艺最佳提取条件为：酶处理温度50 ℃，处理时间2 h，纤维素酶单位活力14 U/mL，琼脂硫酸酯酶单位活力26.6 U/mL，料水比（m/V）为1∶18。在此基础上，分别开展了酶法辅助和复合酶法2种工艺的小试优化结果的验证与200 L放大试验。其中，小试工艺验证结果表明，酶法辅助和复合酶法2种提取工艺下，琼脂的凝胶强度分别为831.9 g/cm2和377.2 g/cm2，得率分别为18.9%和18.3%；200 L中试放大试验结果显示，2种工艺琼脂的凝胶强度分别为1001.3 g/cm2和369.6 g/cm2。说明，2种工艺均具有良好的稳定性和重复性。

Abstract:

In order to reduce alkali consumption in the process of agar extraction,two eco-friendly methods for agar extraction, named enzymeassisted extraction method and composite enzyme extraction method were developed.The extraction conditions were optimized for improvenment of gel strength by singlefactor experiment.The optimized conditions for enzymeassisted process were as follows:3% alkali treatment for 2.5 h,4 U/mL enzyme treatment for 2.5 h at 45 ℃ and pH 7.0.The optimized conditions for composite enzyme was that 14 U/mL cellulase,26.6 U/mL agar sulfatase and mass/water ratio of 1∶18 were used for agar extraction at 50 ℃ for 2 h.The optimal parameters of enzymeassisted and composite enzyme extraction processes were carried out at the 200 L scale in order to validate the feasible of lab procedures.The results showed that the gel strength of agar extracted by enzymeassisted extraction and composite enzyme extraction was 831.9 g/cm2 and 377.2 g/cm2,respectively.The yields were 18.9% and 18.3%, respectively.The results displayed that the gel strengths were 1001.3 g/cm2 and 369.6 g/cm2,respectively,which revealed that both processes have good stability and reproducibility.

参考文献/References:

相似文献/References:

[1]王萍萍,倪辉,姜泽东,等.离子色谱法测定常见海藻及琼脂中的硫酸根含量[J].集美大学学报(自然科学版),2016,21(1):29.
　WANG Ping-ping,NI Hui,JIANG Ze-dong,et al.Analysis of Sulfate Contents in Common Seaweeds and Agars by Ion Chromatography[J].Journal of Jimei University,2016,21(1):29.
[2]张金丽,陈子和,陈燕婷,等.鱼鳞明胶-琼脂-AgNPs复合膜对枇杷的保鲜效果[J].集美大学学报(自然科学版),2020,25(6):411.
　ZHANG Jinli,CHEN Zihe,CHEN Yanting,et al.Effect of Fish Scale Gelatin-Agar Composite Film Incorporated with Silver Nanoparticles on Preservation Quality of Loquat Fruits[J].Journal of Jimei University,2020,25(1):411.
[3]谢杉玉,万博恺,朱艳冰,等.江蓠琼脂碱法提取工艺模型的建立及应用[J].集美大学学报(自然科学版),2022,27(1):45.
　XIE Shanyu,WAN Bokai,ZHU Yanbing,et al.Establishment and Application of the Mathematical Model for Extracting Agar from Gracilaria japonica Using Alkali Treatment[J].Journal of Jimei University,2022,27(1):45.
[4]段金明,吴雨莲,王贺超,等.扩散渗析回收琼脂生产废碱液工艺的响应面法优化[J].集美大学学报(自然科学版),2024,29(4):321.
　DUAN Jinming,WU Yulian,WANG Hechao,et al.Process Optimization for Alkali Recovery from Waste Alkali Liquor in Agar Production Using Diffusion Dialysis by Response Surface Methodology[J].Journal of Jimei University,2024,29(1):321.

备注/Memo

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更新日期/Last Update: 2018-03-20