杂色鲍幼虫变态及TR干扰下内参基因的筛选-《集美大学学报（自然科学版）》

文章信息/Info

Title:: The Stability Comparison of Reference Genes in Metamorphosis and TR RNAi of Haliotis diversicolor Larvae

作者:: 张丽莉1; 2; 徐建波1; 2; 王国栋1; 2; 王艺磊1; 2; （1.集美大学水产学院，福建厦门 361021；2.农业部东海海水健康养殖重点实验室，福建厦门 361021)

Author(s):: ZHANG Lili1; 2; XU Jianbo1; 2; ＷＡＮＧＧuodong1; 2; ＷＡＮＧＹilei1; 2; (1．Fisheries College，Jimei University，Xiamen 361021，China;2．Key Laboratory of Healthy Mariculture for the East China Sea，Ministry of Agriculture，Xiamen 361021，China)

Abstract:: The stability of reference gene strongly impacts the results of real time quantitative PCR（RT-qPCR).The stability of reference gene was analyzed by GeNorm，normFinder and RefFinder in larvae during metamorphosis or under TR RNAi.EIF5A，OAZ1，YB1，RPL3，RPS9 and ACT were choosed as candidate reference genes.The Ct of candidate reference gene was calculated to evaluate its stability by the three softwares.The stability of EIF5A was the highest and ranked the 1st during metamorphosis，which means EIF5A is a good reference gene during metamorphosis，However,its stability decreased in larvae under TR RNAi.On the contrary，RPS9 had a worst stability of the six candidate reference genes during metamorphosis，and had a best stability in larvae under TR RNAi.Only ACT had a good stability and its stability ranked 2nd in metamorphosis and TR RNAi.This mean that ACT is a universal reference gene.The stabilities of other genes also had dramatic changes between metamorphosis and TR RNAi.These results meanthat a gene’s stability has a strong tie with the treatment of samples.In order to ensure accurate results of RT-qPCR，each reference gene should be analyzed in a specific experiment.