Cr6+和Ni2+对拟康宁木霉的毒性及其抗性机制-《集美大学学报（自然科学版）》

文章信息/Info

Title:: Toxicity of Cr6+ and Ni2+ for Trichoderma koningiopsis and Its Resistance Mechanisms

作者:: 林锦美1; 段金明1; 葛晶晶2; 陈锦芳1; 占易苍3; （1.集美大学食品与生物工程学院，福建厦门 361021；2.青岛市环境保护宣传教育中心，山东青岛 266003；3.厦门三达膜环境股份有限公司，福建厦门 361021）

Author(s):: LIN Jinmei1; DUAN Jinming1; GE Jingjing2; CHEN Jinfang1; ZHAN Yicang3; (1.College of Food and Biological Engineering,Jimei University,Xiamen 361021,China;2.Qingdao Environmental Protection and Education Center,Qingdao 266003,China；3.Xiamen Suntar Membrane CO.,LTD,Xiamen 361021,China)

Keywords:: Trichoderma koningiopsis; Cr6+; Ni2+; superoxide dismutase; glutathione; resistance

摘要:: 以实验室筛选到的拟康宁木霉（Trichoderma koningiopsis）为研究对象，探讨了重金属Cr6+和Ni2+胁迫对其菌体生长的影响，并以菌体内的谷胱甘肽(glutathione，GSH)与超氧化物歧化酶（superoxide dismutase，SOD）来表征重金属对菌体的毒性及其抗氧化性。同时，研究了不同浓度外源NO和SO2对重金属胁迫作用下菌体的抗性性能。结果表明，随着Cr6+和Ni2+初始浓度的增加，重金属对拟康宁木霉的毒性增加，并能够诱导菌体内GSH的合成从而抵抗重金属对菌体的毒性，而Cr6+胁迫下，菌量与GSH呈现显著的负相关性，Ni2+胁迫下，菌量与GSH的负相关性不显著。低浓度外源NO能够缓解重金属胁迫下对菌体的氧化损伤作用，GSH与SOD表现出较显著的正相关性（P<0.05）；高浓度NO则加重重金属对菌体的损伤作用，GSH与SOD相关性不显著。在不同浓度外源SO2作用下，菌体的生长均受到抑制，外源SO2加剧重金属对菌体的损伤作用。

Abstract:: The effect of heavy metal Cr6+ and Ni2+ stress on growth of Trichoderma koningiopsis was investigated and the mechanism of microbial resistance to heavy metals was explored based on glutathione (GSH) content and superoxide dismutase (SOD) changes in T.koningiopsis.At the same time,the effect of different concentrations of exogenous NO and SO2 on microbial resistance for heavy metal stress were studied as well.The results showed that,with the increase of the initial concentration of Cr6+ and Ni2+,the growth of T.koningiopsis reduced gradually,and the content of GSH in T.koningiopsis was increased firstly and then decreased.There was a significant negative correlation between GSH content and Cr6+ concentration.The low concentration of exogenous NO could promote the synthesis of GSH and the activity of SOD in T.koningiopsis,while high level of NO could inhibit this phenomenon.The results showed a significant positive correlation between GSH and SOD (P<0.05) under the low concentration of exogenous NO.Different concentration of foreign SO2,inhibited the growth of T.koningiopsis and increased the damage to it.