|本期目录/Table of Contents|

[1]王健,王玲,鲁康乐,等.摄食全豆粕蛋白饲料的牛蛙肠道微生物组成[J].集美大学学报(自然科学版),2018,23(4):241-248.
　WANG Jian,WANG Ling,LU Kangle,et al.An Analysis of the Gut Microbial Community of Bullfrog （Rana(Lithobates) catesbeiana）Fed with Soybean Meal Based Diets[J].Journal of Jimei University,2018,23(4):241-248.
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《集美大学学报（自然科学版）》[ISSN:1007-7405/CN:35-1186/N]

卷:
第23卷
期数:
2018年第4期
页码:
241-248
栏目:
水产、食品与生物工程
出版日期:
2018-07-28

文章信息/Info

Title:
An Analysis of the Gut Microbial Community of Bullfrog （Rana(Lithobates) catesbeiana）Fed with Soybean Meal Based Diets
作者:
王健; 王玲; 鲁康乐; 宋凯; 张春晓
（厦门市饲料检测与安全评价重点实验室，集美大学水产学院，福建 厦门 361021）
Author(s):
WANG Jian; WANG Ling; LU Kangle; SONG Kai; ZHANG Chunxiao
(Key Laboratory for Feed Quality Testing and Safety Evaluation & Fisheries College，Jimei University，Xiamen 361021，China)
关键词:
牛蛙; 16S rDNA; 肠粘膜; 肠内容物; 微生物菌群
Keywords:
bullfrog; 16S rDNA; intestinal mucosa layer; intestinal digesta; microbial community
分类号:
-
DOI:
-
文献标志码:
A
摘要:
以豆粕为主要蛋白源制作牛蛙饲料，饲喂56 d后，采集牛蛙肠粘膜（DC）及肠腔内容物（DN）样品，利用16S rDNA测序技术，对其肠道微生物组成进行分析。结果如下：1）摄食全豆粕蛋白饲料的牛蛙肠道优势菌群为变形菌（Proteobacteria）、梭杆菌（Fusobacteria）和厚壁菌（Firmicutes）。2）DN组微生物丰度指数和多样性指数大于DC组，但未具显著差异性（P＞0.05），而DC组内样品微生物组成的重复性好于DN组。3）DN组在门分类水平优势菌群为变形菌（75%）、厚壁菌（15%）和梭杆菌（5%），在属分类水平上不具规律性；DC组在门分类水平上优势菌群为具有分解纤维能力梭杆菌（78%）和变形菌（20%），在属分类水平上为具发酵利用碳水化合物的鲸杆菌属（Cetobacterium）75%。4）牛蛙肠粘膜上定植一定比例的埃希氏菌-志贺氏菌属（Escherichia-Shigella）和爱德华氏菌属(Edwardsiella)。以上结果表明，牛蛙肠粘膜更适合作为评价饲料对肠道菌群影响的组织，而牛蛙肠粘膜上高比例的梭杆菌门菌可能是牛蛙能够高效利用植物蛋白原料的原因之一。
Abstract:
In this study，bullfrog (Rana(Lithobates)catesbeiana) were fed with soybean meal based diets.After feeding for 56 d,the intestinal mucosa layer (DC) and the intestinal digesta (DN) samples of the bullfrog((133.56±0.14)g)were collected，then the microbial community was analyzed by the 16S rDNA sequencing technology.The results are as follows:1) The most dominant bacterial phyla were Proteobacteria，Fusobacteria，and Firmicutes.2) The microbial abundance index and diversity index of DN were larger than those in DC，however，there was no significant difference between the two sample units (P>0.05).The bacterial taxonomic composition showed better repeatability among all DC samples than that among all DN samples.3) At phylum level，the dominant bacterial in DN were Proteobacteria(75%)，Firmicutes(15%) and Fusobacteria(5%)，while there was no obvious reqularity at genus level.In DC,the dominant bacteria were Fusobacteria(78%) and Proteobacteria(20%).At phylum level,and at genus level，the most dominant bacteria was Cetobacterium,which was able to fement and utilize carbohydrates.4)It also found some Escherichia-Shigella and Edwardsiella colonized the intestinal mucosa layer.These results suggest that the intestinal mucosa layer of bullfrog is more suitable for evaluating the influence of feed on microbial community，and the high abundance of Fusobacteria and Cetobacterium in bullfrog＇s intestinal mucosa layer maybe a reason for that bullfrogs can use plant ingredients efficiently.

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备注/Memo

备注/Memo:
更新日期/Last Update: 2018-09-13