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《集美大学学报（自然科学版）》[ISSN:1007-7405/CN:35-1186/N]

卷:

第24卷

期数:

2019年第4期

页码:

258-266

栏目:

水产、食品与生物工程

出版日期:

2019-07-28

文章信息/Info

Title:

Effects of Freezing Temperature and Duration on Digestion Characteristics of Abalone (Haliotis discus hannai) and Its ACE Inhibitory Activity

作者:

万楚君1; 谢雪琼1; 张凌晶1; 2; 翁凌1; 2; 孙乐常1; 2; 曹敏杰1; 2

(1.集美大学食品与生物工程学院，福建 厦门361021；2.水产品深加工技术国家地方联合工程研究中心，福建 厦门 361021）

Author(s):

WAN Chujun1; XIE Xueqiong1; ZHANG Lingjing1; 2; WENG Ling1; 2; SUN Lechang1; 2; CAO Minjie1; 2

(1.College of Food and Biological Engineering,Jimei University,Xiamen 361021,China;2.National & Local Joint Engineering Research Center of Processing Technology for Aquatic Products,Xiamen 361021,China)

关键词:

皱纹盘鲍; 冻藏温度; 冻藏时间; 模拟胃肠液; 消化;  ACE抑制活性

Keywords:

Haliotis discus hannai; freezing temperature; freezing time; simulated gastrointestinal fluid; digestion; ACE inhibitory activity

分类号:

-

DOI:

-

文献标志码:

A

摘要:

以皱纹盘鲍(Haliotis discus hannai)为研究对象，采用3种冻藏温度（-18，-30，-80 ℃）和3个冻藏时间（3，6，12个月）对鲍鱼肌肉进行冷冻处理，采用十二烷基硫酸钠-聚丙烯酰胺凝胶电泳（sodium dodecyl sulfate-polyacrylamide gel electrophoresis，SDS-PAGE），分析比较了不同冻藏方式下鲍鱼肌肉模拟胃肠液消化的产物特性，利用扫描电镜观察了鲍鱼冷藏过程中肌肉微观结构的变化，研究了各种冻藏鲍鱼消化终产物对血管紧张素转换酶（angiotensin converting enzyme，ACE）的抑制活性。结果表明：胃蛋白酶、胰蛋白酶及胰凝乳蛋白酶对冻藏鲍鱼肌肉蛋白均能分解，但冻藏时间越长，鲍鱼肌肉越难被降解，其消化速率为：3个月>6个月>12个月。但是，不同温度下冻藏相同时间对鲍鱼的消化速率无显著差异。扫描电镜观察发现，冻藏时间对鲍鱼肌肉组织结构影响较大，冻藏3个月的鲍鱼肌肉纤维间隙比新鲜鲍鱼的大，但继续延长冻藏时间至6，12个月，肌肉蛋白凝集，间隙减小。不同冻藏温度和时间的鲍鱼肌肉胃肠液消化产物的ACE抑制活性顺序为：3个月>6个月>12个月，且不同冻藏温度下贮藏鲍鱼消化产物的ACE抑制活性无显著差异。

Abstract:

Three freezing temperatures (-18,-30,-80 ℃) and three freezing durations (3,6,12 months) were designed for abalone (Haliotis discus hannai) muscular protein change investigation during storage.Simulated gastric fluid (SGF) as well as simulated intestinal fluid (SIF) was used to study the effect of in vitro digestion and the changes were evaluated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE).Scanning electronic microscopy (SEM) was employed to observe the changes of muscle microstructures during abalone storage.Angiotensin I-converting enzyme (ACE) inhibitory activity of the digested products was analyzed.The results showed that muscular proteins of abalone could be effectively digested by pepsin,trypsin and chymotrypsin.However,it would be more difficult for samples stored for longer durations and the digestion rates were 3 months > 6 months > 12 months.In addition,no obvious difference in the digestion rate was observed for samples stored at different temperatures for same time intervals.SEM study revealed that freezing duration had a greater impact on the tissue structures.The muscle tissue gaps of abalone stored for 3 months was wider than that of fresh abalone while muscular fiber aggregation and gap diminishing was observed after storage for 6 or 12 months.The ACE inhibitory activities of the gastrointestinal fluids digested products of abalones stored at different freezing temperatures and durations were in the following orders:3 months>6 months>12 months.No significant difference in the ACE inhibitory activity of the digested products of abalones stored at different freezing temperatures was detected.

参考文献/References:

相似文献/References:

[1]赵春暖,严正凛.皱纹盘鲍与九孔鲍杂交的研究[J].集美大学学报(自然科学版),2014,19(5):339.
　ZHAO Chun-nuan,YAN Zheng-lin.Hybridization Between Haliotis discus hannai and Haliotis diversicolor aquatilis[J].Journal of Jimei University,2014,19(4):339.
[2]段雪昆,杜翠红,胡健健,等.皱纹盘鲍Hdh-MMP-1基因cDNA的克隆及原核表达[J].集美大学学报(自然科学版),2016,21(5):321.
　DUAN Xue-kun,DU Cui-hong,HU Jian-jian,et al.cDNA Cloning and Prokaryotic Expression of Matrix Metalloproteinase-1 from Haliotis discus hannai[J].Journal of Jimei University,2016,21(4):321.
[3]胡健健,陈玉磊,段雪昆,等.皱纹盘鲍亮氨酸氨肽酶基因cDNA克隆及表达分析[J].集美大学学报(自然科学版),2017,22(5):31.
　HU Jianjian,CHEN Yulei,DUAN Xuekun,et al.Fulllength cDNA Cloning and Expression Analysis of a Leucine Aminopeptidase from Haliotis discus hannai[J].Journal of Jimei University,2017,22(4):31.
[4]欧俊新,严正凛.南方鲍藻混养技术研究及其效益分析[J].集美大学学报(自然科学版),2011,16(3):172.
　OU Jun-xin,YAN Zheng-lin.The Technology and Efficiency of MacrophyteHaliotis Mixed Breeding Mode in South China[J].Journal of Jimei University,2011,16(4):172.
[5]李越,颜龙杰,翁凌,等.圆二色谱法分析金属离子对鲍鱼脯氨酰内肽酶的作用[J].集美大学学报(自然科学版),2019,24(3):167.
　LI Yue,YAN Longjie,WENG Ling,et al.Effect of Metal Ions on the Activity of a Prolyl Endopeptidase from Haliotis discus hannai Using Circular Dichroism Spectrum[J].Journal of Jimei University,2019,24(4):167.
[6]陈玉磊,李婉玉,吴裕玲,等.皱纹盘鲍基质金属蛋白酶基因的克隆与表达分析[J].集美大学学报(自然科学版),2020,25(1):16.
　CHEN Yulei,LI Wanyu,WU Yuling,et al.Molecular Cloning and Expression Analysis of Matrix Metalloproteinases from Abalone(Haliotis discus hannai)[J].Journal of Jimei University,2020,25(4):16.

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更新日期/Last Update: 2019-08-31