秋刀鱼肉中产胺菌的分离、鉴定及产胺能力分析-《集美大学学报（自然科学版）》

文章信息/Info

Title:: Isolation,Identification and Biogenic Amine Producing Ability Analysis of Biogenic Amine -forming Bacteria from Cololabis saira

Author(s):: LIU Hong; TIAN Xin; LIANG Yiwei; ZHANG Lingjing; CAO Minjie; LIU Guangming; (College of Food and Biological Engineering, Jimei University,Xiamen 361021, China)

Keywords:: Cololabis saira; biogenic amines; biogenic amines-forming bacteria; separation identification; biogenic amine producing ability

摘要:: 为了探究秋刀鱼（Cololabis saira）肉中生物胺产生菌的产胺特性，有效控制生物胺含量，采用改良的Niven’s生物胺筛选培养基，通过16S rDNA序列分析和VITEK 2 Compact System，对秋刀鱼肉中产胺菌进行分离鉴定，分析不同温度（4、25 ℃）条件下产胺菌的产胺能力及生物胺含量变化。结果显示，从秋刀鱼肉中分离鉴定的14株产胺菌分别为摩氏摩根菌、产酸克雷伯菌、阿氏肠杆菌、弗氏柠檬酸杆菌、植生拉乌尔菌、抗坏血酸克吕沃尔菌、粘质沙雷菌、日沟维肠杆菌、肠杆菌属、变性杆菌属、中间克吕沃尔菌、彭氏变形杆菌、拉氏普罗威登斯菌、蜂窝哈夫尼亚菌；在4 ℃条件下，所有产胺菌产胺能力均较弱（11.55~244.75 mg/L）；在25 ℃条件下，产酸克雷伯菌、肠杆菌属的产组胺能力较强，分别为9 889.75，2 891.15 mg/L。结果提示，可通过抑制产酸克雷伯菌、肠杆菌属生长控制秋刀鱼肉中组胺等生物胺的含量。

Abstract:: In order to find an effective way to control the content of biogenic amines in Cololabis.saira，biogenic amine-forming bacteria were isolated and identified by the biogenic amine-forming bacteria screening medium (modified Niven’s agar medium)，16S rDNA sequence and VITEK 2 Compact System.Their abilities of producing biogenic amine at different temperatures (4，25 ℃) were measured，and the changes of the content of the biogenic amine in the C.saira at different temperatures (4，25 ℃) were investigated.The results showed that a total of 14 strains of biogenic amine-forming bacteria were isolated and purified from C.saira，they were identified as Morganella morganii，Klebsiella oxytoca，Enterobacter asburiaede，Citrobacter freundii，Raoultella planticola，Kluyvera ascorbata，Serratia marcescens，Enterobacter gergoviae，Enterobacter sp. ，Proteus sp. ，Kluyvera intermedia，Proteus penneri，Providencia rustigianii，Hafnia alvei，respectively.All of the biogenic amines-forming bacteria had a weak biogenic amine producing ability (11.55-244.75 mg/L) at 4 ℃，while Klebsiella oxytoca and Enterobacter sp.had a strong ability to produce histamine (9 889.75 and 2 891.15 mg/L) at 25 ℃.The results indicated that the content of biogenic amines such as histamine in C.saira might be controlled by inhibiting the growth of Klebsiella oxytoca and Enterobacter sp.