|本期目录/Table of Contents|

[1]朱阳阳,张梦,叶坤,等.17β-雌二醇对大黄鱼性别分化相关基因表达的影响[J].集美大学学报(自然科学版),2019,24(6):401-408.
　ZHU Yangyang,ZHANG Meng,YE Kun,et al.Effects of 17-estradiol on the Expression of Genes Related to Sex Differentiation of the Large Yellow Croaker[J].Journal of Jimei University,2019,24(6):401-408.
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17β-雌二醇对大黄鱼性别分化相关基因表达的影响(PDF)
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《集美大学学报（自然科学版）》[ISSN:1007-7405/CN:35-1186/N]

卷:
第24卷
期数:
2019年第6期
页码:
401-408
栏目:
水产、食品与生物工程
出版日期:
2019-11-28

文章信息/Info

Title:
Effects of 17-estradiol on the Expression of Genes Related to Sex Differentiation of the Large Yellow Croaker
作者:
朱阳阳1; 2; 张梦1; 2; 叶坤1; 2; 沈伟良3; 吴雄飞3; 王志勇1; 2
(1.集美大学水产学院,福建 厦门 361021;2. 农业部东海海水健康养殖重点实验室,福建 厦门 361021 ;3.宁波市海洋与渔业研究院,浙江 宁波 315103 )
Author(s):
ZHU Yangyang1; 2; ZHANG Meng1; 2; YE Kun1; 2; SHEN Weiliang3; WU Xiongfei3; WANG Zhiyong1; 2
(1.Fisheries College,Jimei University,Xiamen 361021,China;2.Key Laboratory of Healthy Mariculture for the East China SeaMinistry of AgricultureJimei UniversityXiamen 361021China;3.Ningbo Institute of Ocean and Fishery,Ningbo 315103,China)
关键词:
大黄鱼; 雌二醇; 性别分化; 基因表达
Keywords:
large yellow croaker(Larimichthys crocea); estradiol; sex differentiation; gene expression
分类号:
-
DOI:
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文献标志码:
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摘要:
为了解17β-雌二醇（E2）对大黄鱼性别决定与性腺发育相关基因表达的影响，在鱼苗培育到41 dph(days post-hatch)时分别投喂添加了0、80、120 mg/kg 17β-雌二醇的配合饲料，并在开始饲喂前（41 dph）和开始饲喂后第30天（70 dph）、第50天（90 dph）、第80天（120 dph）取样，用大黄鱼性别特异分子标记进行遗传性别鉴定后，挑选出遗传雄性（XY）个体，采用qRT-PCR技术检测性腺中amh、gsdf、cyp19a和foxl2四个性别发育与分化相关基因的表达情况。结果表明，E2能下调amh、gsdf、foxl2的表达，对不同发育阶段的大黄鱼体内的性激素转化通路(cyp19a)的影响不同。
Abstract:
The goal of the present study was to investigate the effect of 17β-estradiol(E2) treatment on the expression of genes related to sex determination and gonadal development in the large yellow croaker.When the fry were grown to 41dph(days post-hatch)，they were fed with compound feed containing 0，80 and 120 mg/kg 17β-estradiol，and sampled before starting feeding (41 dph) and 30th d (70 dph)，50th d (90 dph)，80th d (120 dph) after starting feeding.After genetic sex identification with sex-specific molecular markers of the large yellow croaker genetic male (XY) individuals were selected,quantitative real-time PCR（qRT-PCR）was used to analyze the expression levels of genes involved in sexual development and differentiation including amh，gsdf，cyp19a and foxl2 in gonads.The results indicated that E2 could down-regulate the expression of amh，gsdf and foxl2，and has different effects on the sex hormone conversion pathway (cyp19a) in the large yellow croakers at different developmental stages.

参考文献/References:

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更新日期/Last Update: 2020-01-01