|本期目录/Table of Contents|

[1]肖益群,翟少伟,郭松林.鳗鲡源创伤弧菌的间接ELISA快速检测[J].集美大学学报(自然科学版),2020,25(2):81-87.
　XIAO Yiqun,ZHAI Shaowei,GUO Songlin.The Indirect ELISA for Rapid Detection of Vibrio vulnificus from Eels[J].Journal of Jimei University,2020,25(2):81-87.
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《集美大学学报（自然科学版）》[ISSN:1007-7405/CN:35-1186/N]

卷:
第25卷
期数:
2020年第2期
页码:
81-87
栏目:
水产、食品与生物工程
出版日期:
2020-03-28

文章信息/Info

Title:
The Indirect ELISA for Rapid Detection of Vibrio vulnificus from Eels
作者:
肖益群1; 2; 翟少伟1; 2; 郭松林1; 2
(1.集美大学水产学院,福建 厦门 361021;2. 鳗鲡现代产业技术教育部工程研究中心,福建 厦门 361021)
Author(s):
XIAO Yiqun1; 2; ZHAI Shaowei1; 2; GUO Songlin1; 2
(1.Fisheries College,Jimei University,Xiamen 361021,China;2.Engineering Research Center of the Modern Industry Technology for Eel,Ministry of Education,Jimei University,Xiamen 361021,China)
关键词:
美洲鳗鲡; 兔抗血清; 创伤弧菌; 间接ELISA
Keywords:
Auguilla rostrata; rabbit antiserum; Vibrio vulnificus; indirect ELISA
分类号:
-
DOI:
-
文献标志码:
-
摘要:
创伤弧菌(Vibrio vulnificus)血清2型对鳗鲡有专一的感染性，是鳗鲡出血性败血症、烂鳃病和烂尾病的重要病原菌。采用间接ELISA法对制备的兔抗创伤弧菌多克隆抗血清进行效价测定，结果表明其效价高达1∶512 000。从发病鳗鲡中分离出创伤弧菌和37株其他致病菌，将38株病原菌以较低浓度（1×106 cfu/mL ）包被后通过兔抗创伤弧菌血清进行交叉反应水平检测，结果表明，兔抗创伤弧菌血清稀释到1∶256 000或1∶128 000时，其与37株其他病原菌均未产生明显的ELISA交叉反应。该血清分别经1∶64 000和1∶32 000稀释后，采用ELISA方法检测了经创伤弧菌、鳗鲡爱德华氏菌（Edwardsiella anguillarum）和嗜水气单胞菌（Aeromonas hydrophila）攻毒感染死亡的美洲鳗鲡（Auguilla rostrata）脏器组织悬液（肝脏、肾脏和鳃），结果表明，兔抗创伤弧菌血清经1∶32 000稀释后可特异性检出鳗鲡鳃和肾脏组织中的创伤弧菌。
Abstract:
Serum type 2 of Vibrio vulnificus is highly pathogenic to eel and cause hemorrhagic sepsis,gill rot and tail rot.In this study, V.vulnificus and 37 strains of pathogenic bacteria isolated from infected eel were used as bacterial strains.The polyclonal antiserum titers of rabbit anti- V.vulnificus were determined by indirect ELISA,and the results showed that the titer of the antiserum was 1 ∶512 000.Thirty-eight strains of pathogenic bacteria were coated with the concentration of 1×106 cfu/mL and then cross-reacted with the antiserum.The results showed that when the antiserum was diluted to 1 ∶256 000 or 1∶128 000,there was no obvious ELISA cross-reaction between the V.vulnificus and other 37 bacterial pathogens in eels.After the antiserum was diluted at 1 ∶64 000 and 1∶32 000 respectively,the suspensions of liver,kidney and gill of Auguilla rostrata infected by V.vulnificus,E.anguillarum and A.hydrophila were detected by ELISA.The results showed that the 1 ∶32 000 diluted antiserum specifically detect the bacterial pathogen of V.vulnificus in the gill and kidney of eels.

参考文献/References:

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相似文献/References:

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备注/Memo

备注/Memo:
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更新日期/Last Update: 2020-05-22